Modulation of type β transforming growth factor activity in bone cultures by osteotropic hormones

J. Pfeilschifter, G. R. Mundy

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Type β transforming growth factor (TGF-β) activity was found in conditioned medium harvested from fetal rat and neonatal mouse calvariae by using the anchorage-independent growth of normal rat kidney fibroblasts as an indicator system. Calvariae incubated with parathyroid hormone, 1,25-dihydroxyvitamin D3, and interleukin 1, all factors that stimulate bone resorption, showed a concentration-dependent increase in TGF-β activity in the culture medium. Increases in TGF-β activity persisted undiminished for 48 hr after removal of these factors. The increases in TGF-β activity from the resorbing bone cultured were relatively greater than the increases in bone resorption. Calcitonin inhibition of bone resorption correlated with a decrease in TGF-β activity. Thus, agents that modulate bone resorption also affect TGF-β activity in the bone culture medium. Changes in local concentrations of TGF-β activity by osteotropic hormones may be important in the regulation of normal bone remodeling.

Original languageEnglish (US)
Pages (from-to)2024-2028
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number7
StatePublished - 1987
Externally publishedYes

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