Modulation of oxidative stability of haemoglobin inside liposome-encapsulated haemoglobin

Vibhudutta Awasthi, Vivek R. Yadav, Beth Goins, William T. Phillips

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

The major hurdle in the formulation of liposome-encapsulated haemoglobin (LEH) is the oxidation of haemoglobin (Hb) into methaemoglobin during storage and after administration. In order to reduce this oxidative degradation, we tested various reducing conditions in the presence of catalase. We found that at 37°C more than 50% of Hb oxidized to methaemoglobin within 24, whereas in presence of catalase, the oxidation was significantly reduced. The effect of catalase was further enhanced by a reduction mixture containing α-NAD, d-glucose, adenine, inosine, MgCl2, KCl, KH2PO4 and Na2HPO4; only 14% methaemoglobin was generated in the presence of catalase and reduction mixture. Contrary to the expectation, glutathione, deferoxamine and homocysteine enhanced Hb oxidation. The presence of CRM inside liposomes (250m) significantly decreased Hb oxidation. The results suggest that catalase and a well-defined mixture of co-factors may help control Hb oxidation for improvement in the functional life of LEH.

Original languageEnglish (US)
Pages (from-to)471-478
Number of pages8
JournalJournal of Microencapsulation
Volume30
Issue number5
DOIs
StatePublished - 2013

Keywords

  • Catalase
  • Haemoglobin
  • Liposomes
  • Methaemoglobin
  • Oxygen carriers
  • Transfusion

ASJC Scopus subject areas

  • Bioengineering
  • Pharmaceutical Science
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Colloid and Surface Chemistry

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