TY - JOUR
T1 - Modulation of HIV-1 replication by a novel RhoA effector activity
AU - Wang, Liping
AU - Zhang, Hangchun
AU - Solski, Patricia A.
AU - Hart, Matthew J.
AU - Der, Channing J.
AU - Su, Lishan
PY - 2000/5/15
Y1 - 2000/5/15
N2 - The RhoA GTPase is involved in regulating actin cytoskeletal organization, gene expression, cell proliferation, and survival. We report here that p115-RhoGEF, a specific guanine nucleotide exchange factor (GEF) and activator of RhoA, modulates HIV-1 replication. Ectopic expression of p115-RhoGEF or Gα13, which activates p115-RhoGEF activity, leads to inhibition of HIV-1 replication. RhoA activation is required and the inhibition affects HIV-1 gene expression. The RhoA effector activity in inhibiting HIV-1 replication is genetically separable from its activities in transformation of NIH3T3 cells, activation of serum response factor, and actin stress fiber formation. These findings reveal that the RhoA signal transduction pathway regulates HIV-1 replication and suggest that RhoA inhibits HIV-1 replication via a novel effector activity.
AB - The RhoA GTPase is involved in regulating actin cytoskeletal organization, gene expression, cell proliferation, and survival. We report here that p115-RhoGEF, a specific guanine nucleotide exchange factor (GEF) and activator of RhoA, modulates HIV-1 replication. Ectopic expression of p115-RhoGEF or Gα13, which activates p115-RhoGEF activity, leads to inhibition of HIV-1 replication. RhoA activation is required and the inhibition affects HIV-1 gene expression. The RhoA effector activity in inhibiting HIV-1 replication is genetically separable from its activities in transformation of NIH3T3 cells, activation of serum response factor, and actin stress fiber formation. These findings reveal that the RhoA signal transduction pathway regulates HIV-1 replication and suggest that RhoA inhibits HIV-1 replication via a novel effector activity.
UR - http://www.scopus.com/inward/record.url?scp=0034657956&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034657956&partnerID=8YFLogxK
U2 - 10.4049/jimmunol.164.10.5369
DO - 10.4049/jimmunol.164.10.5369
M3 - Article
C2 - 10799900
AN - SCOPUS:0034657956
VL - 164
SP - 5369
EP - 5374
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 10
ER -