Modulation of HIV-1 replication by a novel RhoA effector activity

Liping Wang, Hangchun Zhang, Patricia A. Solski, Matthew J. Hart, Channing J. Der, Lishan Su

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

The RhoA GTPase is involved in regulating actin cytoskeletal organization, gene expression, cell proliferation, and survival. We report here that p115-RhoGEF, a specific guanine nucleotide exchange factor (GEF) and activator of RhoA, modulates HIV-1 replication. Ectopic expression of p115-RhoGEF or Gα13, which activates p115-RhoGEF activity, leads to inhibition of HIV-1 replication. RhoA activation is required and the inhibition affects HIV-1 gene expression. The RhoA effector activity in inhibiting HIV-1 replication is genetically separable from its activities in transformation of NIH3T3 cells, activation of serum response factor, and actin stress fiber formation. These findings reveal that the RhoA signal transduction pathway regulates HIV-1 replication and suggest that RhoA inhibits HIV-1 replication via a novel effector activity.

Original languageEnglish (US)
Pages (from-to)5369-5374
Number of pages6
JournalJournal of Immunology
Volume164
Issue number10
DOIs
StatePublished - May 15 2000
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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