Modulation of food intake by mTOR signalling in the dorsal motor nucleus of the vagus in male rats: Focus on ghrelin and nesfatin-1

Weizhen Zhang, Chao Zhang, Danielle Fritze, Biaoxin Chai, Jiyao Li, Michael W. Mulholland

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Previous studies have demonstrated that mammalian target of rapamycin (mTOR) signalling in the hypothalamus is involved in the control of energy homeostasis. The aim of this study was to characterize the effect of mTOR signalling in the dorsal motor nucleus of the vagus (DMNV) on energy intake. Phospho-mTOR was detected in the DMNV neurons, and its levels were increased by energy deprivation. Rapamycin significantly inhibited mTOR activity and reduced food intake when administrated into the fourth ventricle. Exposure of DMNV neurons to ghrelin increased the phosphorylation of mTOR. Injection of ghrelin into the fourth ventricle significantly increased food intake relative to the control vehicle. Pretreatment with rapamycin for 15 min attenuated the orexigenic effect of ghrelin. A reduction in the phosphorylation of mTOR was observed following injection of nesfatin-1 into the fourth ventricle. When administrated by injection into the fourth ventricle, nesfatin-1 suppressed food intake in comparison with the control vehicle. The anorexigenic effect of nesfatin-1 was significantly attenuated by pretreatment with leucine for 15 min. All these findings suggest that mTOR signalling in the DMNV neurons regulates both the nutrient and the hormonal signals for the modulation of food intake.

Original languageEnglish (US)
Pages (from-to)1696-1704
Number of pages9
JournalExperimental Physiology
Volume98
Issue number12
DOIs
StatePublished - Dec 2013
Externally publishedYes

ASJC Scopus subject areas

  • Physiology
  • Nutrition and Dietetics
  • Physiology (medical)

Fingerprint Dive into the research topics of 'Modulation of food intake by mTOR signalling in the dorsal motor nucleus of the vagus in male rats: Focus on ghrelin and nesfatin-1'. Together they form a unique fingerprint.

Cite this