Modulation of DNA binding properties of CCAAT/enhancer binding protein epsilon by heterodimer formation and interactions with NFkappaB pathway

Alexey M. Chumakov, Agnes Silla, Elizabeth A. Williamson, H. Phillip Koeffler

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

C/EBP epsilon is a transcription factor involved in myeloid cell differentiation. Along with C/EBP-α, -β, -γ, -δ, and -ζ, C/EBP-ε- belongs to the family of CCAAT/enhancer binding proteins that are implicated in control of growth and differentiation of several cell lineages in inflammation and stress response. We have previously shown that C/EBP-ε preferentially binds DNA as a heterodimer with other C/EBP family members such as C/EBP-δ, CHOP (C/EBP-ζ), and the b-zip family protein ATF4. In this study, we define the consensus binding sites for C/EBP-ε dimers and C/EBP-ε-ATF4 heterodimers. We show that the activated NFkappaB pathway promotes interaction of the C/EBP-ε subunit with its cognate DNA binding site via interaction with RelA. RelA-C/EBP interaction is enhanced by phosphorylation of threonine at amino acid 75 and results in increased DNA binding compared with the wild-type nonphosphorylated C/EBP both in vitro and in vivo. We suggest that interaction of the activated NFkappaB pathway and C/EBP-ε- may be important in selective activation of a subset of C/EBP-ε-responsive genes.

Original languageEnglish (US)
Pages (from-to)4209-4219
Number of pages11
JournalBlood
Volume109
Issue number10
DOIs
StatePublished - May 15 2007
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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