Modulation of antioxidant enzymes and programmed cell death by n-3 fatty acids

Gabriel Fernandes, Bysani Chandrasekar, Xianghong Luan, Dean A. Troyer

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Studies from our laboratory indicate that n-3 (fish oil, FO) lipids at 10% (w/w) in a nutritionally adequate, semipurified diet, and supplemented with equal levels of antioxidants, extended the life span of lupus-prone (NZB/NZW)F1 (B/W) female mice as compared to n-6 (corn oil, CO) lipids. The early rise of autoimmune disease in CO-fed mice was closely linked to the loss of T-cell function. Both IL-2 production and IL-2 receptor expression were reduced due to the loss of naive T-Cells and a rise in memory T-cells. Proliferative response to both mitogens and superatigens (staphylococcal enterotoxins A and B) was higher in FO-fed 6.5-mon-old mice. These changes paralleled decreased PGE2 production by splenic cells from FO-fed mice. Analysis of mRNA expression in different organs revealed differential effects of dietary lipids. In FO-fed, mice, transforming growth factor β1 (TGF β1) expression was decreased in kidneys, but splenic tissues had higher expression of TGF β mRNA. As TGF β promotes programmed cell death (PCD), we studied the effects of CO and FO on PCD rates in lymphocytes. Both propidium iodide staining and DNA fragmentation were elevated in lymphocytes of FO-fed mice when compared to CO-fed mice of similar age. Also, increased PCD correlated closely with increased Fas gene expression. Thus, in addition to various other antiinflammatory effects, dietary FO appears to increase PCD and prevent accumulation of self-reactive immune cells in lymphoid organs. Further studies are required to dissect the proand antiinflammatory mechanisms associated with dietary n-3 and n-6 lipids in modulating autoimmune disorders or malignancy during aging.

Original languageEnglish (US)
JournalLipids
Volume31
Issue number1
DOIs
StatePublished - Mar 1996

Fingerprint

Fish Oils
Omega-3 Fatty Acids
Cell death
fish oils
omega-3 fatty acids
Cell Death
apoptosis
Antioxidants
Modulation
Corn Oil
antioxidants
corn oil
mice
Enzymes
enzymes
T-cells
Lipids
Lymphocytes
T-lymphocytes
interleukin-2

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Food Science
  • Organic Chemistry
  • Cell Biology

Cite this

Modulation of antioxidant enzymes and programmed cell death by n-3 fatty acids. / Fernandes, Gabriel; Chandrasekar, Bysani; Luan, Xianghong; Troyer, Dean A.

In: Lipids, Vol. 31, No. 1, 03.1996.

Research output: Contribution to journalArticle

Fernandes, G, Chandrasekar, B, Luan, X & Troyer, DA 1996, 'Modulation of antioxidant enzymes and programmed cell death by n-3 fatty acids', Lipids, vol. 31, no. 1. https://doi.org/10.1007/BF02637058
Fernandes, Gabriel ; Chandrasekar, Bysani ; Luan, Xianghong ; Troyer, Dean A. / Modulation of antioxidant enzymes and programmed cell death by n-3 fatty acids. In: Lipids. 1996 ; Vol. 31, No. 1.
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