Modified associative learning T-maze test for zebrafish (Danio rerio) and other small teleost fish

Research output: Chapter in Book/Report/Conference proceedingChapter

23 Scopus citations


Associative learning is a form of classical (Pavlovian) conditioning in which a neutral stimulus (e.g., the color green) is paired with a stimulus of some significance to an animal (e.g., food), such that for the animal, the color becomes synonymous with food to evoke the same innate, reflexive behavioral responses (e.g., food seeking). This protocol is designed to test the acquisition and extinction of reward-visual stimulus association in zebrafish (Danio rerio) in a T-maze. It is based on the visual discrimination protocol of Colwill et al. (2005), in which colors or patterns are paired with a food reward. The protocol has been modified to include a reward box within the T-maze aquarium so that the influence of drug reinforcement can be studied without the potential confound of reward residues contaminating the testing arena.

Original languageEnglish (US)
Title of host publicationZebrafish Neurobehavioral Protocols
EditorsAllan Kalueff, Jonathan Cachat
Number of pages13
StatePublished - 2011

Publication series

ISSN (Print)0893-2336
ISSN (Electronic)1940-6045


  • Classical conditioning
  • T-maze test
  • acquisition
  • extinction
  • response
  • stimulus

ASJC Scopus subject areas

  • Psychiatry and Mental health
  • General Pharmacology, Toxicology and Pharmaceutics
  • General Biochemistry, Genetics and Molecular Biology
  • General Neuroscience


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