Modeling the dependence of the period of intracellular Ca2+ waves on SERCA expression

Martin Falcke, Yun Li, James D. Lechleiter, Patricia Camacho

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

Contrary to intuitive expectations, overexpression of sarco-endoplasmic reticulum (ER) Ca2+ ATPases (SERCAs) in Xenopus oocytes leads to a decrease in the period and an increase in the amplitude of intracellular Ca 2+ waves. Here we examine these experimental findings by modeling Ca2+ release using a modified Othmer-Tang-model. An increase in the period and a reduction in the amplitude of Ca2+ wave activity are obtained when increases in SERCA density are simulated while keeping all other parameters of the model constant. However, Ca2+ wave period can be reduced and the wave amplitude and velocity can be significantly increased when an increase in the luminal ER Ca2+ concentration due to SERCA overexpression is incorporated into the model. Increased luminal Ca 2+ occurs because increased SERCA activity lowers cytosolic Ca 2+, which is partially replenished by Ca2+ influx across the plasma membrane. These simulations are supported by experimental data demonstrating higher luminal Ca2+ levels, decreased periods, increased amplitude, and increased velocity of Ca2+ waves in response to increased SERCA density.

Original languageEnglish (US)
Pages (from-to)1474-1481
Number of pages8
JournalBiophysical Journal
Volume85
Issue number3
DOIs
StatePublished - Sep 1 2003

ASJC Scopus subject areas

  • Biophysics

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