MMP-9 expression after metallic stent placement in patients with colorectal cancer: Association with in-stent restenosis

Eun Young Kim, Ho Young Song, Jin Cheon Kim, Yong Sik Yoon, Byong Duk Ye, Deok Ho Nam, Su Jin Shin

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Purpose: To verify the expression of matrix metalloproteinase (MMP)-9 in stent-induced hyperplastic tissue from patients with colorectal cancer who received colorectal stents as a bridge to surgery. Materials and Methods: This prospective study was institutional review board- approved, and informed consent was obtained from all patients. Eleven patients (nine men, two women; mean age, 67 years; age range, 53-82 years) with malignant colorectal obstructions who received a colorectal stent between May and December 2010 were included. Tissue specimens were analyzed for MMP-9 and MMP-2 expression. After resection, the tissue was segmented into three parts: tumor tissue, stent-induced tissue hyperplasia, and normal colon tissue. MMP-9 and MMP-2 expression were determined by using zymography, Western blot analysis, and real-time reverse-transcription (qRT) polymerase chain reaction (PCR). Significance of differences between groups was evaluated with Friedman analysis of variance test. Signed-rank test was used to determine differences between malignant tumor tissue and stent-induced hyperplastic tissue groups. Results: Stent placement was technically successful in all 11 patients. Stent-induced hyperplastic tissues were found in all patients. Zymography (P =.003) and Western blot analysis (P =.008) showed that expression of MMP-9 was higher in malignant tumor tissue and stent-induced hyperplastic tissue groups compared with normal colorectal tissue group, demonstrating significant differences between groups but no significant differences between malignant tumor and stent-induced hyperplastic tissues. As for results of qRT PCR analysis, the stent-induced hyperplastic tissue group showed increases in messenger RNA expression level of MMP-9 compared with the malignant tumor tissue group (50.42-fold ± 66.30 higher). Conclusion: High expression of MMP-9 is closely associated with stentinduced colorectal tissue hyperplasia in patients with colorectal cancer.

Original languageEnglish (US)
Pages (from-to)901-908
Number of pages8
JournalRadiology
Volume271
Issue number3
DOIs
StatePublished - Jun 2014
Externally publishedYes

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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