MKL1 defines the H3K4Me3 landscape for NF-κB dependent inflammatory response

Liming Yu, Fei Fang, Xin Dai, Huihui Xu, Xiaohong Qi, Mingming Fang, Yong Xu

Research output: Contribution to journalArticlepeer-review

45 Scopus citations

Abstract

Macrophage-dependent inflammatory response is considered a pivotal biological process that contributes to a host of diseases when aberrantly activated. The underlying epigenetic mechanism is not completely understood. We report here that MKL1 was both sufficient and necessary for p65-dependent pro-inflammatory transcriptional program in immortalized macrophages, in primary human and mouse macrophages, and in an animal model of systemic inflammation (endotoxic shock). Extensive chromatin immunoprecipitation (ChIP) profiling and ChIP-seq analyses revealed that MKL1 deficiency erased key histone modifications synonymous with transactivation on p65 target promoters. Specifically, MKL1 defined histone H3K4 trimethylation landscape for NF-κB dependent transcription. MKL1 recruited an H3K4 trimethyltransferase SET1 to the promoter regions of p65 target genes. There, our work has identified a novel modifier of p65-dependent pro-inflammatory transcription, which may serve as potential therapeutic targets in treating inflammation related diseases.

Original languageEnglish (US)
Article number191
JournalScientific reports
Volume7
Issue number1
DOIs
StatePublished - 2017
Externally publishedYes

ASJC Scopus subject areas

  • General

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