Abstract
In a survey of enzymes related to protein oxidation and cellular redox state, we found activity of the redox enzyme thioredoxin reductase (TXNRD) to be elevated in cells from long-lived species of rodents, primates, and birds. Elevated TXNRD activity in long-lived species reflected increases in the mitochondrial form, TXNRD2, rather than the cytosolic forms TXNRD1 and TXNRD3. Analysis of published RNA-Seq data showed elevated TXNRD2 mRNA in multiple organs of longer-lived primates, suggesting that the phenomenon is not limited to skin-derived fibroblasts. Elevation of TXNRD2 activity and protein levels was also noted in liver of three different long-lived mutant mice, and in normal male mice treated with a drug that extends lifespan in males. Overexpression of mitochondrial TXNRD2 in Drosophila melanogaster extended median (but not maximum) lifespan in female flies with a small lifespan extension in males; in contrast, overexpression of the cytosolic form, TXNRD1, did not produce a lifespan extension.
Original language | English (US) |
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Pages (from-to) | 683-692 |
Number of pages | 10 |
Journal | Aging cell |
Volume | 16 |
Issue number | 4 |
DOIs | |
State | Published - Aug 2017 |
Keywords
- Drosophila
- TXN
- TXNRD2
- Trxr2
- aging
- lifespan
- mice
- mitochondria
- oxidative stress
- primates
- rodents
- thioredoxin
ASJC Scopus subject areas
- Aging
- Cell Biology