Mitochondrial common deletion is elevated in blood of breast cancer patients mediated by oxidative stress

Hezhongrong Nie, Guorong Chen, Jing He, Fengjiao Zhang, Ming Li, Qiufeng Wang, Huaibin Zhou, Jianxin Lyu, Yidong Bai

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

The 4977 bp common deletion is one of the most frequently observed mitochondrial DNA (mtDNA) mutations in human tissues and has been implicated in various human cancer types. It is generally believed that continuous generation of intracellular reactive oxygen species (ROS) during oxidative phosphorylation (OXPHOS) is a major underlying mechanism for generation of such mtDNA deletions while antioxidant systems, including Manganese superoxide dismutase (MnSOD), mitigating the deleterious effects of ROS. However, the clinical significance of this common deletion remains to be explored. A comprehensive investigation on occurrence and accumulation of the common deletion and mtDNA copy number was carried out in breast carcinoma (BC) patients, benign breast disease (BBD) patients and age-matched healthy donors in our study. Meanwhile, the representative oxidative (ROS production, mtDNA and lipid oxidative damage) and anti-oxidative features (MnSOD expression level and variation) in blood samples from these groups were also analyzed. We found that the mtDNA common deletion is much more likely to be detected in BC patients at relatively high levels while the mtDNA content is lower. This alteration has been associated with a higher MnSOD level and higher oxidative damages in both BC and BBD patients. Our results indicate that the mtDNA common deletion in blood may serve a biomarker for the breast cancer.

Original languageEnglish (US)
Pages (from-to)104-112
Number of pages9
JournalMitochondrion
Volume26
DOIs
StatePublished - Jan 1 2016
Externally publishedYes

Keywords

  • 4977 bp common deletion
  • Breast cancer
  • MnSOD
  • MtDNA mutations
  • Oxidative stress

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Molecular Medicine

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