@article{17554d673e23447e8239a50b43a0aba3,
title = "Mitochondrial Ca2+ Uniporter Is a Mitochondrial Luminal Redox Sensor that Augments MCU Channel Activity",
abstract = "Ca2+ dynamics and oxidative signaling are fundamental mechanisms for mitochondrial bioenergetics and cell function. The MCU complex is the major pathway by which these signals are integrated in mitochondria. Whether and how these coactive elements interact with MCU have not been established. As an approach toward understanding the regulation of MCU channel by oxidative milieu, we adapted inflammatory and hypoxia models. We identified the conserved cysteine 97 (Cys-97) to be the only reactive thiol in human MCU that undergoes S-glutathionylation. Furthermore, biochemical, structural, and superresolution imaging analysis revealed that MCU oxidation promotes MCU higher order oligomer formation. Both oxidation and mutation of MCU Cys-97 exhibited persistent MCU channel activity with higher [Ca2+]m uptake rate, elevated mROS, and enhanced [Ca2+]m overload-induced cell death. In contrast, these effects were largely independent of MCU interaction with its regulators. These findings reveal a distinct functional role for Cys-97 in ROS sensing and regulation of MCU activity.",
keywords = "EMRE, MCU, MCUR1, MICU1, MICU2, bioenergetics, calcium, gluathionylation, mitochondria, reactive oxygen species",
author = "Zhiwei Dong and Santhanam Shanmughapriya and Dhanendra Tomar and Naveed Siddiqui and Solomon Lynch and Neeharika Nemani and Breves, {Sarah L.} and Xueqian Zhang and Aparna Tripathi and Palaniappan Palaniappan and Riitano, {Massimo F.} and Worth, {Alison M.} and Ajay Seelam and Edmund Carvalho and Ramasamy Subbiah and Fabi{\'a}n Ja{\~n}a and Jonathan Soboloff and Yizhi Peng and Cheung, {Joseph Y.} and Joseph, {Suresh K.} and Jeffrey Caplan and Sudarsan Rajan and Stathopulos, {Peter B.} and Muniswamy Madesh",
note = "Funding Information: This research was funded by the NIH (R01GM109882, R01HL086699, R01HL119306, and 1S10RR027327 to M.M. and P01 DA037830 [principal investigator, K. Khalili] and RO1DK103558 to S.K.J.). Z.D. is supported by China Scholarship Council (201403170252). S.L. is supported by the NIH (R01GM109882 supplement). Access to super-resolution microscopy was supported by NIH-NIGMS (P20 GM103446), NSF (IIA-1301765), and the State of Delaware. F.J. is supported by FONDECYT postdoctoral fellowship 3140458. S.S. is supported by the AHA post-doctoral fellowship (17POST33410414) and the Natural Sciences and Engineering Research Council of Canada (05239), and the Canadian Foundation for Innovation/Ontario Research Fund (34113) provided support to P.B.S. Publisher Copyright: {\textcopyright} 2017 Elsevier Inc.",
year = "2017",
month = mar,
day = "16",
doi = "10.1016/j.molcel.2017.01.032",
language = "English (US)",
volume = "65",
pages = "1014--1028.e7",
journal = "Molecular Cell",
issn = "1097-2765",
publisher = "Cell Press",
number = "6",
}