Migration of co-cultured endothelial cells and osteoblasts in composite hydroxyapatite/polylactic acid scaffolds

Amita R. Shah, Sarita R. Shah, Sunho Oh, Joo L. Ong, Joseph C. Wenke, C. Mauli Agrawal

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Regeneration of bone in large segmental bone defects requires regeneration of both cortical bone and trabecular bone. A scaffold design consisting of a hydroxyapatite (HA) ring surrounding a polylactic acid (PLA) core simulates the structure of bone and provides an environment for indirect and direct co-culture conditions. In this experiment, human umbilical vein endothelial cells (EC) and normal human primary osteoblasts (OB) were co-cultured to evaluate cell migration and interactions within this biphasic composite scaffold. Both cell types were able to migrate between the different material phases of the scaffold. It was also observed that OB migration increased when they were co-cultured with ECs, whereas EC migration decreased in co-culture. The results show that co-culture of ECs and OBs in this composite biphasic scaffold allows for migration of cells throughout the scaffold and that pre-seeding a scaffold with ECs can increase OB infiltration into desired areas of the scaffold.

Original languageEnglish (US)
Pages (from-to)2501-2509
Number of pages9
JournalAnnals of Biomedical Engineering
Volume39
Issue number10
DOIs
StatePublished - Oct 1 2011

Keywords

  • Bone regeneration
  • Ceramic scaffold
  • Endothelial cell migration
  • Osteoblast migration
  • Polymer scaffold

ASJC Scopus subject areas

  • Biomedical Engineering

Fingerprint Dive into the research topics of 'Migration of co-cultured endothelial cells and osteoblasts in composite hydroxyapatite/polylactic acid scaffolds'. Together they form a unique fingerprint.

Cite this