TY - JOUR
T1 - Microtubule dynamics in vitro are regulated by the tubulin isotype composition
AU - Panda, Dulal
AU - Miller, Herbert P.
AU - Banerjee, Asok
AU - Ludueña, Richard F.
AU - Wilson, Leslie
PY - 1994/11/22
Y1 - 1994/11/22
N2 - The growing and shortening dynamics of individual bovine brain microtubules at their plus ends at steady state in vitro, assembled from isotypically pure αβ(II), αβ(III), or αβ(IV) tubulin dimers, were determined by differential interference contrast video microscopy. Microtubules assembled from the purified αβ(III) isotype were considerably more dynamic than microtubules made from the αβ(II) or αβ(IV) isotypes or from unfractionated phosphocellulose-purified tubulin. Furthermore, increasing the proportion of the αβ(II) isotype in a mixture of the αβ(II) and αβ(III) isotypes suppressed microtubule dynamics, demonstrating that microtubule dynamics can be influenced by the tubulin isotype composition. The data support the hypothesis that cells might determine the dynamic properties and functions of its microtubules in part by altering the relative amounts of the different tubulin isotypes.
AB - The growing and shortening dynamics of individual bovine brain microtubules at their plus ends at steady state in vitro, assembled from isotypically pure αβ(II), αβ(III), or αβ(IV) tubulin dimers, were determined by differential interference contrast video microscopy. Microtubules assembled from the purified αβ(III) isotype were considerably more dynamic than microtubules made from the αβ(II) or αβ(IV) isotypes or from unfractionated phosphocellulose-purified tubulin. Furthermore, increasing the proportion of the αβ(II) isotype in a mixture of the αβ(II) and αβ(III) isotypes suppressed microtubule dynamics, demonstrating that microtubule dynamics can be influenced by the tubulin isotype composition. The data support the hypothesis that cells might determine the dynamic properties and functions of its microtubules in part by altering the relative amounts of the different tubulin isotypes.
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U2 - 10.1073/pnas.91.24.11358
DO - 10.1073/pnas.91.24.11358
M3 - Article
C2 - 7972064
AN - SCOPUS:0028113390
VL - 91
SP - 11358
EP - 11362
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 24
ER -