Microsphere and dilution techniques for the determination of blood flows and volumes in conscious mice

R. W. Barbee, B. D. Perry, R. N. Re, Joseph P Murgo

Research output: Contribution to journalArticle

121 Citations (Scopus)

Abstract

Although the mouse is the most commonly used transgenic species, little is known regarding cardiovascular and fluid homeostasis in this animal. Therefore, the reference microsphere and dilution techniques were adapted for the measurement of cardiac output (CO), regional blood flows, and intravascular fluid volumes in the conscious mouse. Previously acclimatized C3H mice were studied 4-5 h after surgery and recovery from anesthesia. Approximately 40,000 85Sr-labeled microspheres were injected into the left ventricle while a reference sample was withdrawn at one of two rates from the femoral artery. 51Cr and 125I were used for the determination of blood volume (BV), plasma volume (PV), and F(cells) ratio (whole body hematocrit/large vessel hematocrit). CO and BV in the conscious mouse were 16 ± 1.4 ml/min and 2.3 ± 0.1 ml, respectively. Anesthesia lowered heart rate, blood pressure, PV, and altered the distribution of CO. Two successive injections of 15,000-20,000 microspheres were tolerated in the mouse without an increase in total peripheral resistance. The results indicate that the microsphere and indicator dilution techniques can be applied to study cardiovascular and fluid homeostasis in the mouse.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume263
Issue number3 32-3
StatePublished - 1992
Externally publishedYes

Fingerprint

Blood Volume Determination
Indicator Dilution Techniques
Microspheres
Cardiac Output
Plasma Volume
Hematocrit
Homeostasis
Anesthesia
Cardiac Volume
Inbred C3H Mouse
Regional Blood Flow
Femoral Artery
Blood Volume
Vascular Resistance
Heart Ventricles
Heart Rate
Blood Pressure
Injections

Keywords

  • blood volume determination
  • cardiac output
  • hematocrit
  • mammals
  • plasma volume
  • stroke volume

ASJC Scopus subject areas

  • Physiology

Cite this

Microsphere and dilution techniques for the determination of blood flows and volumes in conscious mice. / Barbee, R. W.; Perry, B. D.; Re, R. N.; Murgo, Joseph P.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 263, No. 3 32-3, 1992.

Research output: Contribution to journalArticle

@article{79cb53b5f3014660940307838eeac886,
title = "Microsphere and dilution techniques for the determination of blood flows and volumes in conscious mice",
abstract = "Although the mouse is the most commonly used transgenic species, little is known regarding cardiovascular and fluid homeostasis in this animal. Therefore, the reference microsphere and dilution techniques were adapted for the measurement of cardiac output (CO), regional blood flows, and intravascular fluid volumes in the conscious mouse. Previously acclimatized C3H mice were studied 4-5 h after surgery and recovery from anesthesia. Approximately 40,000 85Sr-labeled microspheres were injected into the left ventricle while a reference sample was withdrawn at one of two rates from the femoral artery. 51Cr and 125I were used for the determination of blood volume (BV), plasma volume (PV), and F(cells) ratio (whole body hematocrit/large vessel hematocrit). CO and BV in the conscious mouse were 16 ± 1.4 ml/min and 2.3 ± 0.1 ml, respectively. Anesthesia lowered heart rate, blood pressure, PV, and altered the distribution of CO. Two successive injections of 15,000-20,000 microspheres were tolerated in the mouse without an increase in total peripheral resistance. The results indicate that the microsphere and indicator dilution techniques can be applied to study cardiovascular and fluid homeostasis in the mouse.",
keywords = "blood volume determination, cardiac output, hematocrit, mammals, plasma volume, stroke volume",
author = "Barbee, {R. W.} and Perry, {B. D.} and Re, {R. N.} and Murgo, {Joseph P}",
year = "1992",
language = "English (US)",
volume = "263",
journal = "American Journal of Physiology - Renal Physiology",
issn = "0363-6127",
publisher = "American Physiological Society",
number = "3 32-3",

}

TY - JOUR

T1 - Microsphere and dilution techniques for the determination of blood flows and volumes in conscious mice

AU - Barbee, R. W.

AU - Perry, B. D.

AU - Re, R. N.

AU - Murgo, Joseph P

PY - 1992

Y1 - 1992

N2 - Although the mouse is the most commonly used transgenic species, little is known regarding cardiovascular and fluid homeostasis in this animal. Therefore, the reference microsphere and dilution techniques were adapted for the measurement of cardiac output (CO), regional blood flows, and intravascular fluid volumes in the conscious mouse. Previously acclimatized C3H mice were studied 4-5 h after surgery and recovery from anesthesia. Approximately 40,000 85Sr-labeled microspheres were injected into the left ventricle while a reference sample was withdrawn at one of two rates from the femoral artery. 51Cr and 125I were used for the determination of blood volume (BV), plasma volume (PV), and F(cells) ratio (whole body hematocrit/large vessel hematocrit). CO and BV in the conscious mouse were 16 ± 1.4 ml/min and 2.3 ± 0.1 ml, respectively. Anesthesia lowered heart rate, blood pressure, PV, and altered the distribution of CO. Two successive injections of 15,000-20,000 microspheres were tolerated in the mouse without an increase in total peripheral resistance. The results indicate that the microsphere and indicator dilution techniques can be applied to study cardiovascular and fluid homeostasis in the mouse.

AB - Although the mouse is the most commonly used transgenic species, little is known regarding cardiovascular and fluid homeostasis in this animal. Therefore, the reference microsphere and dilution techniques were adapted for the measurement of cardiac output (CO), regional blood flows, and intravascular fluid volumes in the conscious mouse. Previously acclimatized C3H mice were studied 4-5 h after surgery and recovery from anesthesia. Approximately 40,000 85Sr-labeled microspheres were injected into the left ventricle while a reference sample was withdrawn at one of two rates from the femoral artery. 51Cr and 125I were used for the determination of blood volume (BV), plasma volume (PV), and F(cells) ratio (whole body hematocrit/large vessel hematocrit). CO and BV in the conscious mouse were 16 ± 1.4 ml/min and 2.3 ± 0.1 ml, respectively. Anesthesia lowered heart rate, blood pressure, PV, and altered the distribution of CO. Two successive injections of 15,000-20,000 microspheres were tolerated in the mouse without an increase in total peripheral resistance. The results indicate that the microsphere and indicator dilution techniques can be applied to study cardiovascular and fluid homeostasis in the mouse.

KW - blood volume determination

KW - cardiac output

KW - hematocrit

KW - mammals

KW - plasma volume

KW - stroke volume

UR - http://www.scopus.com/inward/record.url?scp=0026666825&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026666825&partnerID=8YFLogxK

M3 - Article

C2 - 1415664

AN - SCOPUS:0026666825

VL - 263

JO - American Journal of Physiology - Renal Physiology

JF - American Journal of Physiology - Renal Physiology

SN - 0363-6127

IS - 3 32-3

ER -