Microsomal lauric acid 11- and 12-hydroxylation: A new assay method utilizing high pressure liquid chromatography

Lucy L. Fan, Bettie Sue S. Masters, Russell A. Prough

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

A new assay method using high pressure liquid chromatography has been developed which permits the simultaneous isolation, determination, and quantitation of lauric acid and its hydroxylated products after methylation of extracts from kidney or liver microsomal incubation mixtures. The small differences in polarity between the lauric acid, 11-hydroxy- and 12-hydroxy-lauric acid after methylation permit their separation on reverse phase columns packed with octadecyltrichlorosilane bonded to silicone polymers. The total time required for the chromatography is less than 1 hr. Using this method, the formation of hydroxylated products was shown to have a linear dependence on protein concentration and time. The Km for lauric acid and NADPH were determined to be 8 μm and 54 μm in kidney microsomes, respectively.

Original languageEnglish (US)
Pages (from-to)265-272
Number of pages8
JournalAnalytical Biochemistry
Volume71
Issue number1
DOIs
StatePublished - Mar 1976

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Microsomal lauric acid 11- and 12-hydroxylation: A new assay method utilizing high pressure liquid chromatography'. Together they form a unique fingerprint.

  • Cite this