Microsatellite-enriched genomic libraries as a source of polymorphic loci for Schistosoma mansoni: Primer note

N. B. Rodrigues, M. R. Silva, M. M. Pucci, D. J. Minchella, R. Sorensen, P. T. Loverde, A. J. Romanha, G. Oliveira

Research output: Contribution to journalReview articlepeer-review

18 Scopus citations

Abstract

Microsatellite markers for Schistosoma mansoni were developed using four genomic microsatellite-enriched libraries. Microsatellites were observed in 65.4% of all sequences. Primer pairs were designed and tested for 23 loci. Eighteen loci produced amplification products, out of which 11 were polymorphic and were further characterized on 100 individuals of S. mansoni. Two to 19 alleles per locus were detected. The average values of expected and observed heterozygosities among the 11 loci were 0.79 and 0.59, respectively.

Original languageEnglish (US)
Pages (from-to)263-265
Number of pages3
JournalMolecular Ecology Notes
Volume7
Issue number2
DOIs
StatePublished - Mar 1 2007

Keywords

  • Computational biology
  • Genome
  • Genomic library
  • Microsatellites
  • Population genetics
  • Schistosoma mansoni

ASJC Scopus subject areas

  • Biochemistry
  • Ecology
  • Biochemistry, Genetics and Molecular Biology(all)

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