Microsatellite-enriched genomic libraries as a source of polymorphic loci for Schistosoma mansoni: Primer note

N. B. Rodrigues; M. R. Silva; M. M. Pucci; D. J. Minchella; R. Sorensen; P. T. Loverde; A. J. Romanha; G. Oliveira

doi:10.1111/j.1471-8286.2006.01575.x

Microsatellite-enriched genomic libraries as a source of polymorphic loci for Schistosoma mansoni: Primer note

N. B. Rodrigues, M. R. Silva, M. M. Pucci, D. J. Minchella, R. Sorensen, P. T. Loverde, A. J. Romanha, G. Oliveira

Biochemistry & Structural Biology

Research output: Contribution to journal › Review article › peer-review

18 Scopus citations

Abstract

Microsatellite markers for Schistosoma mansoni were developed using four genomic microsatellite-enriched libraries. Microsatellites were observed in 65.4% of all sequences. Primer pairs were designed and tested for 23 loci. Eighteen loci produced amplification products, out of which 11 were polymorphic and were further characterized on 100 individuals of S. mansoni. Two to 19 alleles per locus were detected. The average values of expected and observed heterozygosities among the 11 loci were 0.79 and 0.59, respectively.

Original language	English (US)
Pages (from-to)	263-265
Number of pages	3
Journal	Molecular Ecology Notes
Volume	7
Issue number	2
DOIs	https://doi.org/10.1111/j.1471-8286.2006.01575.x
State	Published - Mar 2007

Keywords

Computational biology
Genome
Genomic library
Microsatellites
Population genetics
Schistosoma mansoni

ASJC Scopus subject areas

Biochemistry
Ecology
Biochemistry, Genetics and Molecular Biology(all)

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Microsatellite-enriched genomic libraries as a source of polymorphic loci for Schistosoma mansoni : Primer note. / Rodrigues, N. B.; Silva, M. R.; Pucci, M. M.; Minchella, D. J.; Sorensen, R.; Loverde, P. T.; Romanha, A. J.; Oliveira, G.

In: Molecular Ecology Notes, Vol. 7, No. 2, 03.2007, p. 263-265.

Research output: Contribution to journal › Review article › peer-review

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abstract = "Microsatellite markers for Schistosoma mansoni were developed using four genomic microsatellite-enriched libraries. Microsatellites were observed in 65.4% of all sequences. Primer pairs were designed and tested for 23 loci. Eighteen loci produced amplification products, out of which 11 were polymorphic and were further characterized on 100 individuals of S. mansoni. Two to 19 alleles per locus were detected. The average values of expected and observed heterozygosities among the 11 loci were 0.79 and 0.59, respectively.",

keywords = "Computational biology, Genome, Genomic library, Microsatellites, Population genetics, Schistosoma mansoni",

author = "Rodrigues, {N. B.} and Silva, {M. R.} and Pucci, {M. M.} and Minchella, {D. J.} and R. Sorensen and Loverde, {P. T.} and Romanha, {A. J.} and G. Oliveira",

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T2 - Primer note

AU - Rodrigues, N. B.

AU - Silva, M. R.

AU - Pucci, M. M.

AU - Minchella, D. J.

AU - Sorensen, R.

AU - Loverde, P. T.

AU - Romanha, A. J.

AU - Oliveira, G.

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N2 - Microsatellite markers for Schistosoma mansoni were developed using four genomic microsatellite-enriched libraries. Microsatellites were observed in 65.4% of all sequences. Primer pairs were designed and tested for 23 loci. Eighteen loci produced amplification products, out of which 11 were polymorphic and were further characterized on 100 individuals of S. mansoni. Two to 19 alleles per locus were detected. The average values of expected and observed heterozygosities among the 11 loci were 0.79 and 0.59, respectively.

AB - Microsatellite markers for Schistosoma mansoni were developed using four genomic microsatellite-enriched libraries. Microsatellites were observed in 65.4% of all sequences. Primer pairs were designed and tested for 23 loci. Eighteen loci produced amplification products, out of which 11 were polymorphic and were further characterized on 100 individuals of S. mansoni. Two to 19 alleles per locus were detected. The average values of expected and observed heterozygosities among the 11 loci were 0.79 and 0.59, respectively.

KW - Computational biology

KW - Genome

KW - Genomic library

KW - Microsatellites

KW - Population genetics

KW - Schistosoma mansoni

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Microsatellite-enriched genomic libraries as a source of polymorphic loci for Schistosoma mansoni: Primer note

Abstract

Keywords

ASJC Scopus subject areas

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