TY - JOUR
T1 - MicroRNA expression profiles differentiate chronic pain condition subtypes
AU - Ciszek, Brittney P.
AU - Khan, Asma A.
AU - Dang, Hong
AU - Slade, Gary D.
AU - Smith, Shad
AU - Bair, Eric
AU - Maixner, William
AU - Zolnoun, Denniz
AU - Nackley, Andrea G.
N1 - Funding Information:
All authors have read the journal''s authorship agreement and policy on disclosure of potential conflicts of interest. Authors have no financial disclosures to make and no conflicts of interest to disclose. This work was funded by National Institute of Health/National Institute of Neurological Disorders (NIH/NINDS) P01 NS045685 to A.N.; NIH Office of Behavioral and Social Sciences Research (OBSSR) R24 DK067674 to A.N. and D.Z.; NIH/NINDS R01 NS072205 to A.N.; and a National Vulvodynia Association (NVA) grant to A.N. and D.Z. The authors thank Lawrence Yoon (GlaxoSmith Kline, Inc, Research Triangle Park, North Carolina) for his assistance with microRNA methods.
Funding Information:
This work was funded by National Institute of Health/National Institute of Neurological Disorders (NIH/NINDS) P01 NS045685 to A.N.; NIH Office of Behavioral and Social Sciences Research (OBSSR) R24 DK067674 to A.N. and D.Z.; NIH / NINDS R01 NS072205 to A.N.; and a National Vulvodynia Association (NVA) grant to A.N. and D.Z.
PY - 2015/12/1
Y1 - 2015/12/1
N2 - Chronic pain is a significant health care problem, ineffectively treated because of its unclear etiology and heterogeneous clinical presentation. Emerging evidence demonstrates that microRNAs (miRNAs) regulate the expression of pain-relevant genes, yet little is known about their role in chronic pain. Here, we evaluate the relationship among pain, psychological characteristics, plasma cytokines, and whole blood miRNAs in 22 healthy controls (HCs); 33 subjects with chronic pelvic pain (vestibulodynia, VBD); and 23 subjects with VBD and irritable bowel syndrome (VBD + IBS). VBD subjects were similar to HCs in self-reported pain, psychological profiles, and remote bodily pain. VBD + IBS subjects reported decreased health and function; and an increase in headaches, somatization, and remote bodily pain. Furthermore, VBD subjects exhibited a balance in proinflammatory and anti-inflammatory cytokines, whereas VBD + IBS subjects failed to exhibit a compensatory increase in anti-inflammatory cytokines. VBD subjects differed from controls in expression of 10 miRNAs of predicted importance for pain and estrogen signaling. VBD + IBS subjects differed from controls in expression of 11 miRNAs of predicted importance for pain, cell physiology, and insulin signaling. miRNA expression was correlated with pain-relevant phenotypes and cytokine levels. These results suggest that miRNAs represent a valuable tool for differentiating VBD subtypes (localized pain with apparent peripheral neurosensory disruption vs widespread pain with a central sensory contribution) that may require different treatment approaches.
AB - Chronic pain is a significant health care problem, ineffectively treated because of its unclear etiology and heterogeneous clinical presentation. Emerging evidence demonstrates that microRNAs (miRNAs) regulate the expression of pain-relevant genes, yet little is known about their role in chronic pain. Here, we evaluate the relationship among pain, psychological characteristics, plasma cytokines, and whole blood miRNAs in 22 healthy controls (HCs); 33 subjects with chronic pelvic pain (vestibulodynia, VBD); and 23 subjects with VBD and irritable bowel syndrome (VBD + IBS). VBD subjects were similar to HCs in self-reported pain, psychological profiles, and remote bodily pain. VBD + IBS subjects reported decreased health and function; and an increase in headaches, somatization, and remote bodily pain. Furthermore, VBD subjects exhibited a balance in proinflammatory and anti-inflammatory cytokines, whereas VBD + IBS subjects failed to exhibit a compensatory increase in anti-inflammatory cytokines. VBD subjects differed from controls in expression of 10 miRNAs of predicted importance for pain and estrogen signaling. VBD + IBS subjects differed from controls in expression of 11 miRNAs of predicted importance for pain, cell physiology, and insulin signaling. miRNA expression was correlated with pain-relevant phenotypes and cytokine levels. These results suggest that miRNAs represent a valuable tool for differentiating VBD subtypes (localized pain with apparent peripheral neurosensory disruption vs widespread pain with a central sensory contribution) that may require different treatment approaches.
KW - Abbreviations CCPC complex chronic pain condition
KW - CNS central nervous system
KW - FM fibromyalgia
KW - HC healthy controls
KW - IBS irritable bowel syndrome
KW - IL-1ra interleukin 1 receptor antagonist
KW - IL-8 interleukin 8
KW - TMD temporomandibular disorder
KW - TMJ temporomandibular joint
KW - VBD vestibulodynia
KW - miRNAs microRNAs
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U2 - 10.1016/j.trsl.2015.06.008
DO - 10.1016/j.trsl.2015.06.008
M3 - Article
C2 - 26166255
AN - SCOPUS:84960471674
VL - 166
SP - 706-720.e11
JO - Translational Research
JF - Translational Research
SN - 1931-5244
IS - 6
ER -