Utilizing a method of in vitro microperfusion of the duct of the human eccrine sweat gland, the sweat electrolyte abnormality of cystic fibrosis was studied. In one set of experiments, sweat ducts from subjects without cystic fibrosis were perfused with isotonic sweat from either patients with cystic fibrosis or from matching controls. The bidirectional fluxes of sodium and the net fluxes of water and potassium were determined. The net reabsorption and back diffusion of sodium were significantly smaller during perfusion with sweat from patients than with sweat from controls. The net efflux of water and influx of potassium were not different. In another set of experiments, sweat ducts were obtained from patients with cystic fibrosis immediately after death and were microperfused with sweat from control subjects. The abnormalities in the transductal fluxes of cation were similar to those found in sweat ducts from control subjects perfused with sweat from patients with cystic fibrosis. It is concluded that the eccrine sweat gland abnormality of cystic fibrosis is due to a sodium transport inhibitory effect exerted intraluminally by the sweat of affected persons.
|Original language||English (US)|
|Number of pages||13|
|Journal||Texas Reports on Biology and Medicine|
|State||Published - Dec 1 1973|
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