Micro/nanorough titanium-aluminum-vanadium alloy surfaces trigger alternate osteoblast integrin expression profile

R. A. Gittens, K. H. Sandhage, R. Olivares-Navarrete, S. L. Hyzy, Zvi Schwartz, B. D. Boyan

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Recent studies of new surface modifications that superimpose well-defined nanostructures on microrough implants mimicking the hierarchical complexity of native bone, report synergistically enhanced osteoblast maturation and local factor production at the protein level compared to smooth, nanorough and microrough surfaces. Whether these micro/nanorough surfaces enhance the osteogenic response by triggering similar patterns of integrin receptors and their associated signaling pathways as with well-established microrough surfaces is not well understood. Human osteoblasts (hOBs) were cultured until confluent for gene expression studies on tissue culture polystyrene (TCPS) or on titanium alloy (Ti6Al4V) disks with different surface topography: smooth, nanorough, microrough and micro/nanorough surfaces. mRNA expression levels of osteogenesis-related markers such as osteocalcin (BGLAP) and bone sialoprotein (BSP), bone morphogenetic protein 2 (BMP2), BMP4, noggin (NOG) and gremlin 1 (GREM1) were all higher on microrough and micro/nanorough surfaces, with few differences between them, compared to smooth and nanorough groups. Interestingly, expression of integrins α1 and α2, which interact primarily with collagens and laminin and have been commonly associated with osteoblast differentiation on microrough Ti and Ti6Al4V, were expressed at lower levels on micro/nanorough surfaces compared to microrough ones. Conversely, the αv subunit, which binds ligands such as vitronectin, osteopontin, and bone sialoprotein among others, had higher expression on micro/nanorough surfaces concomitantly with regulation of the β3 mRNA levels on nanomodified surfaces. These results suggest that the maturation of osteoblasts on micro/nanorough surfaces may be occurring through different integrin engagement than those established for microrough-only surfaces.

Original languageEnglish (US)
Title of host publicationProceedings of the IEEE Annual Northeast Bioengineering Conference, NEBEC
PublisherInstitute of Electrical and Electronics Engineers Inc.
Volume2014-December
ISBN (Print)9781479937288
DOIs
StatePublished - Dec 2 2014
Externally publishedYes
Event2014 40th Annual Northeast Bioengineering Conference, NEBEC 2014 - Boston, United States
Duration: Apr 25 2014Apr 27 2014

Other

Other2014 40th Annual Northeast Bioengineering Conference, NEBEC 2014
CountryUnited States
CityBoston
Period4/25/144/27/14

Keywords

  • bone
  • gene expression
  • implants
  • mRNA
  • nanostructures
  • osseointegration
  • surface properties

ASJC Scopus subject areas

  • Bioengineering

Fingerprint Dive into the research topics of 'Micro/nanorough titanium-aluminum-vanadium alloy surfaces trigger alternate osteoblast integrin expression profile'. Together they form a unique fingerprint.

  • Cite this

    Gittens, R. A., Sandhage, K. H., Olivares-Navarrete, R., Hyzy, S. L., Schwartz, Z., & Boyan, B. D. (2014). Micro/nanorough titanium-aluminum-vanadium alloy surfaces trigger alternate osteoblast integrin expression profile. In Proceedings of the IEEE Annual Northeast Bioengineering Conference, NEBEC (Vol. 2014-December). [6972798] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/NEBEC.2014.6972798