Micro/nanorough titanium-aluminum-vanadium alloy surfaces trigger alternate osteoblast integrin expression profile

R. A. Gittens, K. H. Sandhage, R. Olivares-Navarrete, S. L. Hyzy, Zvi Schwartz, B. D. Boyan

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Recent studies of new surface modifications that superimpose well-defined nanostructures on microrough implants mimicking the hierarchical complexity of native bone, report synergistically enhanced osteoblast maturation and local factor production at the protein level compared to smooth, nanorough and microrough surfaces. Whether these micro/nanorough surfaces enhance the osteogenic response by triggering similar patterns of integrin receptors and their associated signaling pathways as with well-established microrough surfaces is not well understood. Human osteoblasts (hOBs) were cultured until confluent for gene expression studies on tissue culture polystyrene (TCPS) or on titanium alloy (Ti6Al4V) disks with different surface topography: smooth, nanorough, microrough and micro/nanorough surfaces. mRNA expression levels of osteogenesis-related markers such as osteocalcin (BGLAP) and bone sialoprotein (BSP), bone morphogenetic protein 2 (BMP2), BMP4, noggin (NOG) and gremlin 1 (GREM1) were all higher on microrough and micro/nanorough surfaces, with few differences between them, compared to smooth and nanorough groups. Interestingly, expression of integrins α1 and α2, which interact primarily with collagens and laminin and have been commonly associated with osteoblast differentiation on microrough Ti and Ti6Al4V, were expressed at lower levels on micro/nanorough surfaces compared to microrough ones. Conversely, the αv subunit, which binds ligands such as vitronectin, osteopontin, and bone sialoprotein among others, had higher expression on micro/nanorough surfaces concomitantly with regulation of the β3 mRNA levels on nanomodified surfaces. These results suggest that the maturation of osteoblasts on micro/nanorough surfaces may be occurring through different integrin engagement than those established for microrough-only surfaces.

Original languageEnglish (US)
Title of host publicationProceedings of the IEEE Annual Northeast Bioengineering Conference, NEBEC
PublisherInstitute of Electrical and Electronics Engineers Inc.
Volume2014-December
ISBN (Print)9781479937288
DOIs
StatePublished - Dec 2 2014
Externally publishedYes
Event2014 40th Annual Northeast Bioengineering Conference, NEBEC 2014 - Boston, United States
Duration: Apr 25 2014Apr 27 2014

Other

Other2014 40th Annual Northeast Bioengineering Conference, NEBEC 2014
CountryUnited States
CityBoston
Period4/25/144/27/14

Fingerprint

Vanadium alloys
Osteoblasts
Titanium
Integrins
Aluminum alloys
Bone
Integrin-Binding Sialoprotein
Proteins
Vitronectin
Bone Morphogenetic Protein 2
Tissue culture
Messenger RNA
Osteopontin
Polystyrenes
Osteocalcin
Laminin
Surface topography
Titanium alloys
Collagen
Gene expression

Keywords

  • bone
  • gene expression
  • implants
  • mRNA
  • nanostructures
  • osseointegration
  • surface properties

ASJC Scopus subject areas

  • Bioengineering

Cite this

Gittens, R. A., Sandhage, K. H., Olivares-Navarrete, R., Hyzy, S. L., Schwartz, Z., & Boyan, B. D. (2014). Micro/nanorough titanium-aluminum-vanadium alloy surfaces trigger alternate osteoblast integrin expression profile. In Proceedings of the IEEE Annual Northeast Bioengineering Conference, NEBEC (Vol. 2014-December). [6972798] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/NEBEC.2014.6972798

Micro/nanorough titanium-aluminum-vanadium alloy surfaces trigger alternate osteoblast integrin expression profile. / Gittens, R. A.; Sandhage, K. H.; Olivares-Navarrete, R.; Hyzy, S. L.; Schwartz, Zvi; Boyan, B. D.

Proceedings of the IEEE Annual Northeast Bioengineering Conference, NEBEC. Vol. 2014-December Institute of Electrical and Electronics Engineers Inc., 2014. 6972798.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Gittens, RA, Sandhage, KH, Olivares-Navarrete, R, Hyzy, SL, Schwartz, Z & Boyan, BD 2014, Micro/nanorough titanium-aluminum-vanadium alloy surfaces trigger alternate osteoblast integrin expression profile. in Proceedings of the IEEE Annual Northeast Bioengineering Conference, NEBEC. vol. 2014-December, 6972798, Institute of Electrical and Electronics Engineers Inc., 2014 40th Annual Northeast Bioengineering Conference, NEBEC 2014, Boston, United States, 4/25/14. https://doi.org/10.1109/NEBEC.2014.6972798
Gittens RA, Sandhage KH, Olivares-Navarrete R, Hyzy SL, Schwartz Z, Boyan BD. Micro/nanorough titanium-aluminum-vanadium alloy surfaces trigger alternate osteoblast integrin expression profile. In Proceedings of the IEEE Annual Northeast Bioengineering Conference, NEBEC. Vol. 2014-December. Institute of Electrical and Electronics Engineers Inc. 2014. 6972798 https://doi.org/10.1109/NEBEC.2014.6972798
Gittens, R. A. ; Sandhage, K. H. ; Olivares-Navarrete, R. ; Hyzy, S. L. ; Schwartz, Zvi ; Boyan, B. D. / Micro/nanorough titanium-aluminum-vanadium alloy surfaces trigger alternate osteoblast integrin expression profile. Proceedings of the IEEE Annual Northeast Bioengineering Conference, NEBEC. Vol. 2014-December Institute of Electrical and Electronics Engineers Inc., 2014.
@inproceedings{fc6b72c5832e49b683c36741803bb068,
title = "Micro/nanorough titanium-aluminum-vanadium alloy surfaces trigger alternate osteoblast integrin expression profile",
abstract = "Recent studies of new surface modifications that superimpose well-defined nanostructures on microrough implants mimicking the hierarchical complexity of native bone, report synergistically enhanced osteoblast maturation and local factor production at the protein level compared to smooth, nanorough and microrough surfaces. Whether these micro/nanorough surfaces enhance the osteogenic response by triggering similar patterns of integrin receptors and their associated signaling pathways as with well-established microrough surfaces is not well understood. Human osteoblasts (hOBs) were cultured until confluent for gene expression studies on tissue culture polystyrene (TCPS) or on titanium alloy (Ti6Al4V) disks with different surface topography: smooth, nanorough, microrough and micro/nanorough surfaces. mRNA expression levels of osteogenesis-related markers such as osteocalcin (BGLAP) and bone sialoprotein (BSP), bone morphogenetic protein 2 (BMP2), BMP4, noggin (NOG) and gremlin 1 (GREM1) were all higher on microrough and micro/nanorough surfaces, with few differences between them, compared to smooth and nanorough groups. Interestingly, expression of integrins α1 and α2, which interact primarily with collagens and laminin and have been commonly associated with osteoblast differentiation on microrough Ti and Ti6Al4V, were expressed at lower levels on micro/nanorough surfaces compared to microrough ones. Conversely, the αv subunit, which binds ligands such as vitronectin, osteopontin, and bone sialoprotein among others, had higher expression on micro/nanorough surfaces concomitantly with regulation of the β3 mRNA levels on nanomodified surfaces. These results suggest that the maturation of osteoblasts on micro/nanorough surfaces may be occurring through different integrin engagement than those established for microrough-only surfaces.",
keywords = "bone, gene expression, implants, mRNA, nanostructures, osseointegration, surface properties",
author = "Gittens, {R. A.} and Sandhage, {K. H.} and R. Olivares-Navarrete and Hyzy, {S. L.} and Zvi Schwartz and Boyan, {B. D.}",
year = "2014",
month = "12",
day = "2",
doi = "10.1109/NEBEC.2014.6972798",
language = "English (US)",
isbn = "9781479937288",
volume = "2014-December",
booktitle = "Proceedings of the IEEE Annual Northeast Bioengineering Conference, NEBEC",
publisher = "Institute of Electrical and Electronics Engineers Inc.",
address = "United States",

}

TY - GEN

T1 - Micro/nanorough titanium-aluminum-vanadium alloy surfaces trigger alternate osteoblast integrin expression profile

AU - Gittens, R. A.

AU - Sandhage, K. H.

AU - Olivares-Navarrete, R.

AU - Hyzy, S. L.

AU - Schwartz, Zvi

AU - Boyan, B. D.

PY - 2014/12/2

Y1 - 2014/12/2

N2 - Recent studies of new surface modifications that superimpose well-defined nanostructures on microrough implants mimicking the hierarchical complexity of native bone, report synergistically enhanced osteoblast maturation and local factor production at the protein level compared to smooth, nanorough and microrough surfaces. Whether these micro/nanorough surfaces enhance the osteogenic response by triggering similar patterns of integrin receptors and their associated signaling pathways as with well-established microrough surfaces is not well understood. Human osteoblasts (hOBs) were cultured until confluent for gene expression studies on tissue culture polystyrene (TCPS) or on titanium alloy (Ti6Al4V) disks with different surface topography: smooth, nanorough, microrough and micro/nanorough surfaces. mRNA expression levels of osteogenesis-related markers such as osteocalcin (BGLAP) and bone sialoprotein (BSP), bone morphogenetic protein 2 (BMP2), BMP4, noggin (NOG) and gremlin 1 (GREM1) were all higher on microrough and micro/nanorough surfaces, with few differences between them, compared to smooth and nanorough groups. Interestingly, expression of integrins α1 and α2, which interact primarily with collagens and laminin and have been commonly associated with osteoblast differentiation on microrough Ti and Ti6Al4V, were expressed at lower levels on micro/nanorough surfaces compared to microrough ones. Conversely, the αv subunit, which binds ligands such as vitronectin, osteopontin, and bone sialoprotein among others, had higher expression on micro/nanorough surfaces concomitantly with regulation of the β3 mRNA levels on nanomodified surfaces. These results suggest that the maturation of osteoblasts on micro/nanorough surfaces may be occurring through different integrin engagement than those established for microrough-only surfaces.

AB - Recent studies of new surface modifications that superimpose well-defined nanostructures on microrough implants mimicking the hierarchical complexity of native bone, report synergistically enhanced osteoblast maturation and local factor production at the protein level compared to smooth, nanorough and microrough surfaces. Whether these micro/nanorough surfaces enhance the osteogenic response by triggering similar patterns of integrin receptors and their associated signaling pathways as with well-established microrough surfaces is not well understood. Human osteoblasts (hOBs) were cultured until confluent for gene expression studies on tissue culture polystyrene (TCPS) or on titanium alloy (Ti6Al4V) disks with different surface topography: smooth, nanorough, microrough and micro/nanorough surfaces. mRNA expression levels of osteogenesis-related markers such as osteocalcin (BGLAP) and bone sialoprotein (BSP), bone morphogenetic protein 2 (BMP2), BMP4, noggin (NOG) and gremlin 1 (GREM1) were all higher on microrough and micro/nanorough surfaces, with few differences between them, compared to smooth and nanorough groups. Interestingly, expression of integrins α1 and α2, which interact primarily with collagens and laminin and have been commonly associated with osteoblast differentiation on microrough Ti and Ti6Al4V, were expressed at lower levels on micro/nanorough surfaces compared to microrough ones. Conversely, the αv subunit, which binds ligands such as vitronectin, osteopontin, and bone sialoprotein among others, had higher expression on micro/nanorough surfaces concomitantly with regulation of the β3 mRNA levels on nanomodified surfaces. These results suggest that the maturation of osteoblasts on micro/nanorough surfaces may be occurring through different integrin engagement than those established for microrough-only surfaces.

KW - bone

KW - gene expression

KW - implants

KW - mRNA

KW - nanostructures

KW - osseointegration

KW - surface properties

UR - http://www.scopus.com/inward/record.url?scp=84940707116&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84940707116&partnerID=8YFLogxK

U2 - 10.1109/NEBEC.2014.6972798

DO - 10.1109/NEBEC.2014.6972798

M3 - Conference contribution

AN - SCOPUS:84940707116

SN - 9781479937288

VL - 2014-December

BT - Proceedings of the IEEE Annual Northeast Bioengineering Conference, NEBEC

PB - Institute of Electrical and Electronics Engineers Inc.

ER -