Microencapsulation of stem cells for therapy

Shirae K. Leslie, Ramsey C. Kinney, Zvi Schwartz, Barbara D. Boyan

Research output: Chapter in Book/Report/Conference proceedingChapter

7 Scopus citations

Abstract

An increasing demand to regenerate tissues from patient-derived sources has led to the development of cell-based therapies using autologous stem cells, thereby decreasing immune rejection of scaffolds coupled with allogeneic stem cells or allografts. Adult stem cells are multipotent and are readily available in tissues such as fat and bone marrow. They possess the ability to repair and regenerate tissue through the production of therapeutic factors, particularly vasculogenic proteins. A major challenge in cell-based therapies is localizing the delivered stem cells to the target site. Microencapsulation of cells provides a porous polymeric matrix that can provide a protected environment, localize the cells to one area, and maintain their viability by enabling the exchange of nutrients and waste products between the encapsulated cells and the surrounding tissue. In this chapter, we describe a method to produce injectable microbeads containing a tunable number of stem cells using the biopolymer alginate. The microencapsulation process involves extrusion of the alginate suspension containing cells from a microencapsulator, a syringe pump to control its flow rate, an electrostatic potential to overcome capillary forces and a reduced Ca ++ cross-linking solution containing a nutrient osmolyte, to form microbeads. This method allows the encapsulated cells to remain viable up to three weeks in culture and up to three months in vivo and secrete growth factors capable of supporting tissue regeneration.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press
Pages251-259
Number of pages9
DOIs
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1479
ISSN (Print)1064-3745

Keywords

  • Alginate
  • Cell-based therapy
  • Degradation
  • Hydrogel
  • Microbeads
  • Microencapsulation
  • Stem cells

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Leslie, S. K., Kinney, R. C., Schwartz, Z., & Boyan, B. D. (2017). Microencapsulation of stem cells for therapy. In Methods in Molecular Biology (pp. 251-259). (Methods in Molecular Biology; Vol. 1479). Humana Press. https://doi.org/10.1007/978-1-4939-6364-5_20