TY - JOUR
T1 - Microbiologic and serologic studies of gardnerella vuginalis in Intra-amniotic infection
AU - Gibbs, Ronald S.
AU - Weiner, Mark H.
AU - Walmer, Katherine
AU - St Clair, Patricia I.
PY - 1987/8
Y1 - 1987/8
N2 - Our objective was to investigate the role of Gardiierella vagIIIalis in intra-amniotic infection by use of comparative, quantitative cultures on selective media and by detection of maternal antibody response. Amniotic fluid was collected from patients with intra-amniotic infection and from matched control women. In addition to media for aerobes, anaerobes, and mycoplasmas, we used V agar-selective (Remel, Lenexa, KS) to isolate G vagIIIalis. Acute and convalescent maternal sera were collected and assayed for antibodies by a microenzyme-linked immunosorbent assay (ELISA) prepared against whole cells of G vagiiralis. Gardirerella vaginalis was isolated in the amniotic fluid of 24 (28%) of the 86 patients with intra-amniotic infection, but this was not significantly different from the isolation rate in amniotic fluid of 86 matched controls (21%). No patient exhibited G vagiiralis bacteremia. The ELISA performed on paired sera of selected patients showed that 25 had intraamniotic infection (eight G vaginalis-positive, 17 negative), and 18 were asymptomatic (seven G vagIIIalis-positive, 11 negative). The amount of G vagIIIalis antibodies detected by ELISA in acute sera was similar in all four groups. Mean changes during convalescence were small (.053-.084 optical density units) and not significantly different. Although G vagIIIalis is found commonly in amniotic fluid of patients with intra-amniotic infection, the data do not support a pathogenic role for this organism; however, a facilitating role in polymicrobial infection cannot be excluded.
AB - Our objective was to investigate the role of Gardiierella vagIIIalis in intra-amniotic infection by use of comparative, quantitative cultures on selective media and by detection of maternal antibody response. Amniotic fluid was collected from patients with intra-amniotic infection and from matched control women. In addition to media for aerobes, anaerobes, and mycoplasmas, we used V agar-selective (Remel, Lenexa, KS) to isolate G vagIIIalis. Acute and convalescent maternal sera were collected and assayed for antibodies by a microenzyme-linked immunosorbent assay (ELISA) prepared against whole cells of G vagiiralis. Gardirerella vaginalis was isolated in the amniotic fluid of 24 (28%) of the 86 patients with intra-amniotic infection, but this was not significantly different from the isolation rate in amniotic fluid of 86 matched controls (21%). No patient exhibited G vagiiralis bacteremia. The ELISA performed on paired sera of selected patients showed that 25 had intraamniotic infection (eight G vaginalis-positive, 17 negative), and 18 were asymptomatic (seven G vagIIIalis-positive, 11 negative). The amount of G vagIIIalis antibodies detected by ELISA in acute sera was similar in all four groups. Mean changes during convalescence were small (.053-.084 optical density units) and not significantly different. Although G vagIIIalis is found commonly in amniotic fluid of patients with intra-amniotic infection, the data do not support a pathogenic role for this organism; however, a facilitating role in polymicrobial infection cannot be excluded.
UR - http://www.scopus.com/inward/record.url?scp=0023265436&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023265436&partnerID=8YFLogxK
M3 - Article
C2 - 3496566
AN - SCOPUS:0023265436
VL - 70
SP - 187
EP - 190
JO - Obstetrics and Gynecology
JF - Obstetrics and Gynecology
SN - 0029-7844
IS - 2
ER -