Methylator resistance mediated by mismatch repair deficiency in a glioblastoma multiforme xenograft

Henry S. Friedman; Stewart P. Johnson; Qing Dong; S. Clifford Schold; B. K.Ahmed Rasheed; Sandra H. Bigner; Francis Ali-Osman; Eileen Dolan; O. Michael Colvin; Peter Houghton; Glen Germain; James T. Drummond; Stephen Keir; Susan Marcelli; Darell D. Bigner; Paul Modrich

Methylator resistance mediated by mismatch repair deficiency in a glioblastoma multiforme xenograft

Henry S. Friedman
, Stewart P. Johnson
, Qing Dong
, S. Clifford Schold
, B. K.Ahmed Rasheed
, Sandra H. Bigner
, Francis Ali-Osman
, Eileen Dolan
, O. Michael Colvin
, Peter Houghton
, Glen Germain
, James T. Drummond
, Stephen Keir
, Susan Marcelli
, Darell D. Bigner
, Paul Modrich

Research output: Contribution to journal › Article › peer-review

Abstract

A methylator-resistant human glioblastoma multiforme xenograft, D-245 MG (PR), in athymic nude mice was established by serially treating the parent xenograft D-245 MG with procarbazine. D-245 MG xenografts were sensitive to procarbazine, temozolomide, N-methyl-N-nitrosourea, 1,3-bis(2-chloroethyl)- 1-nitrosourea, 9-aminocamptothecin, topotecan, CPT-11, cyclophosphamide, and busulfan. D-245 MG (PR) xenografts were resistant to procarbazine, temozolomide, N-methyl-N-nitrosourea, and busulfan, but they were sensitive to the other agents. Both D-245 MG and D-245 MG (PR) xenografts displayed no O⁶-alkylguanine-DNA alkyltransferase activity, and their levels of glutathione and glutathione-S-transferase were similar. D-245 MG xenografts expressed the human mismatch repair proteins hMSH2 and hMLH1, whereas D-245 MG (PR) expressed hMLH1 but not hMSH2.

Original language	English (US)
Pages (from-to)	2933-2936
Number of pages	4
Journal	Cancer Research
Volume	57
Issue number	14
State	Published - Jul 15 1997
Externally published	Yes

ASJC Scopus subject areas

Oncology
Cancer Research

Cite this

Friedman, H. S., Johnson, S. P., Dong, Q., Schold, S. C., Rasheed, B. K. A., Bigner, S. H., Ali-Osman, F., Dolan, E., Colvin, O. M., Houghton, P., Germain, G., Drummond, J. T., Keir, S., Marcelli, S., Bigner, D. D., & Modrich, P. (1997). Methylator resistance mediated by mismatch repair deficiency in a glioblastoma multiforme xenograft. Cancer Research, 57(14), 2933-2936.

@article{98ff3264437243ae92fa2d32fbf17825,

title = "Methylator resistance mediated by mismatch repair deficiency in a glioblastoma multiforme xenograft",

abstract = "A methylator-resistant human glioblastoma multiforme xenograft, D-245 MG (PR), in athymic nude mice was established by serially treating the parent xenograft D-245 MG with procarbazine. D-245 MG xenografts were sensitive to procarbazine, temozolomide, N-methyl-N-nitrosourea, 1,3-bis(2-chloroethyl)- 1-nitrosourea, 9-aminocamptothecin, topotecan, CPT-11, cyclophosphamide, and busulfan. D-245 MG (PR) xenografts were resistant to procarbazine, temozolomide, N-methyl-N-nitrosourea, and busulfan, but they were sensitive to the other agents. Both D-245 MG and D-245 MG (PR) xenografts displayed no O6-alkylguanine-DNA alkyltransferase activity, and their levels of glutathione and glutathione-S-transferase were similar. D-245 MG xenografts expressed the human mismatch repair proteins hMSH2 and hMLH1, whereas D-245 MG (PR) expressed hMLH1 but not hMSH2.",

author = "Friedman, \{Henry S.\} and Johnson, \{Stewart P.\} and Qing Dong and Schold, \{S. Clifford\} and Rasheed, \{B. K.Ahmed\} and Bigner, \{Sandra H.\} and Francis Ali-Osman and Eileen Dolan and Colvin, \{O. Michael\} and Peter Houghton and Glen Germain and Drummond, \{James T.\} and Stephen Keir and Susan Marcelli and Bigner, \{Darell D.\} and Paul Modrich",

year = "1997",

month = jul,

day = "15",

language = "English (US)",

volume = "57",

pages = "2933--2936",

journal = "Cancer Research",

issn = "0008-5472",

publisher = "American Association for Cancer Research Inc.",

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TY - JOUR

T1 - Methylator resistance mediated by mismatch repair deficiency in a glioblastoma multiforme xenograft

AU - Friedman, Henry S.

AU - Johnson, Stewart P.

AU - Dong, Qing

AU - Schold, S. Clifford

AU - Rasheed, B. K.Ahmed

AU - Bigner, Sandra H.

AU - Ali-Osman, Francis

AU - Dolan, Eileen

AU - Colvin, O. Michael

AU - Houghton, Peter

AU - Germain, Glen

AU - Drummond, James T.

AU - Keir, Stephen

AU - Marcelli, Susan

AU - Bigner, Darell D.

AU - Modrich, Paul

PY - 1997/7/15

Y1 - 1997/7/15

N2 - A methylator-resistant human glioblastoma multiforme xenograft, D-245 MG (PR), in athymic nude mice was established by serially treating the parent xenograft D-245 MG with procarbazine. D-245 MG xenografts were sensitive to procarbazine, temozolomide, N-methyl-N-nitrosourea, 1,3-bis(2-chloroethyl)- 1-nitrosourea, 9-aminocamptothecin, topotecan, CPT-11, cyclophosphamide, and busulfan. D-245 MG (PR) xenografts were resistant to procarbazine, temozolomide, N-methyl-N-nitrosourea, and busulfan, but they were sensitive to the other agents. Both D-245 MG and D-245 MG (PR) xenografts displayed no O6-alkylguanine-DNA alkyltransferase activity, and their levels of glutathione and glutathione-S-transferase were similar. D-245 MG xenografts expressed the human mismatch repair proteins hMSH2 and hMLH1, whereas D-245 MG (PR) expressed hMLH1 but not hMSH2.

AB - A methylator-resistant human glioblastoma multiforme xenograft, D-245 MG (PR), in athymic nude mice was established by serially treating the parent xenograft D-245 MG with procarbazine. D-245 MG xenografts were sensitive to procarbazine, temozolomide, N-methyl-N-nitrosourea, 1,3-bis(2-chloroethyl)- 1-nitrosourea, 9-aminocamptothecin, topotecan, CPT-11, cyclophosphamide, and busulfan. D-245 MG (PR) xenografts were resistant to procarbazine, temozolomide, N-methyl-N-nitrosourea, and busulfan, but they were sensitive to the other agents. Both D-245 MG and D-245 MG (PR) xenografts displayed no O6-alkylguanine-DNA alkyltransferase activity, and their levels of glutathione and glutathione-S-transferase were similar. D-245 MG xenografts expressed the human mismatch repair proteins hMSH2 and hMLH1, whereas D-245 MG (PR) expressed hMLH1 but not hMSH2.

UR - https://www.scopus.com/pages/publications/0030834332

UR - https://www.scopus.com/pages/publications/0030834332#tab=citedBy

M3 - Article

C2 - 9230204

AN - SCOPUS:0030834332

SN - 0008-5472

VL - 57

SP - 2933

EP - 2936

JO - Cancer Research

JF - Cancer Research

IS - 14

ER -

Methylator resistance mediated by mismatch repair deficiency in a glioblastoma multiforme xenograft

Abstract

ASJC Scopus subject areas

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