TY - JOUR
T1 - Methylator resistance mediated by mismatch repair deficiency in a glioblastoma multiforme xenograft
AU - Friedman, Henry S.
AU - Johnson, Stewart P.
AU - Dong, Qing
AU - Schold, S. Clifford
AU - Rasheed, B. K.Ahmed
AU - Bigner, Sandra H.
AU - Ali-Osman, Francis
AU - Dolan, Eileen
AU - Colvin, O. Michael
AU - Houghton, Peter
AU - Germain, Glen
AU - Drummond, James T.
AU - Keir, Stephen
AU - Marcelli, Susan
AU - Bigner, Darell D.
AU - Modrich, Paul
PY - 1997/7/15
Y1 - 1997/7/15
N2 - A methylator-resistant human glioblastoma multiforme xenograft, D-245 MG (PR), in athymic nude mice was established by serially treating the parent xenograft D-245 MG with procarbazine. D-245 MG xenografts were sensitive to procarbazine, temozolomide, N-methyl-N-nitrosourea, 1,3-bis(2-chloroethyl)- 1-nitrosourea, 9-aminocamptothecin, topotecan, CPT-11, cyclophosphamide, and busulfan. D-245 MG (PR) xenografts were resistant to procarbazine, temozolomide, N-methyl-N-nitrosourea, and busulfan, but they were sensitive to the other agents. Both D-245 MG and D-245 MG (PR) xenografts displayed no O6-alkylguanine-DNA alkyltransferase activity, and their levels of glutathione and glutathione-S-transferase were similar. D-245 MG xenografts expressed the human mismatch repair proteins hMSH2 and hMLH1, whereas D-245 MG (PR) expressed hMLH1 but not hMSH2.
AB - A methylator-resistant human glioblastoma multiforme xenograft, D-245 MG (PR), in athymic nude mice was established by serially treating the parent xenograft D-245 MG with procarbazine. D-245 MG xenografts were sensitive to procarbazine, temozolomide, N-methyl-N-nitrosourea, 1,3-bis(2-chloroethyl)- 1-nitrosourea, 9-aminocamptothecin, topotecan, CPT-11, cyclophosphamide, and busulfan. D-245 MG (PR) xenografts were resistant to procarbazine, temozolomide, N-methyl-N-nitrosourea, and busulfan, but they were sensitive to the other agents. Both D-245 MG and D-245 MG (PR) xenografts displayed no O6-alkylguanine-DNA alkyltransferase activity, and their levels of glutathione and glutathione-S-transferase were similar. D-245 MG xenografts expressed the human mismatch repair proteins hMSH2 and hMLH1, whereas D-245 MG (PR) expressed hMLH1 but not hMSH2.
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M3 - Article
C2 - 9230204
AN - SCOPUS:0030834332
SN - 0008-5472
VL - 57
SP - 2933
EP - 2936
JO - Cancer Research
JF - Cancer Research
IS - 14
ER -