Methylation microarray analysis of late-stage ovarian carcinomas distinguishes progression-free survival in patients and identifies candidate epigenetic markers

Susan H. Wei, Chuan Mu Chen, Gordon Strathdee, Jaturon Harnsomburana, Chi Ren Shyu, Farahnaz Rahmatpanah, Huidong Shi, Shu Wing Ng, Pearlly S. Yan, Kenneth P. Nephew, Robert Brown, Tim Hui Ming Huang

Research output: Contribution to journalArticle

165 Scopus citations

Abstract

Purpose: The purpose of this study was to profile methylation alterations of CpG islands in ovarian tumors and to identify candidate markers for diagnosis and prognosis of the disease. Experimental Design: A global analysis of DNA methylation using a novel microarray approach called differential methylation hybridization was performed on 19 patients with stage III and IV ovarian carcinomas. Results: Hierarchical clustering identified two groups of patients with distinct methylation profiles. Tumors from group 1 contained high levels of concurrent methylation, whereas group 2 tumors had lower tumor methylation levels. The duration of progression-free survival after chemotherapy was significantly shorter for patients in group 1 compared with group 2 (P < 0.001). Differential methylation in tumors was independently confirmed by methylation-specific PCR. Conclusions: The data suggest that a higher degree of CpG island methylation is associated with early disease recurrence after chemotherapy. The differential methylation hybridization assay also identified a select group of CpG island loci that are potentially useful as epigenetic markers for predicting treatment outcome in ovarian cancer patients.

Original languageEnglish (US)
Pages (from-to)2246-2252
Number of pages7
JournalClinical Cancer Research
Volume8
Issue number7
StatePublished - 2002
Externally publishedYes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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