Methods for the design and analysis of sedimentation velocity and sedimentation equilibrium experiments with proteins

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35 Scopus citations

Abstract

Analytical ultracentrifugation experiments play an integral role in the solution phase characterization of recombinant proteins and other biological macromolecules. This unit discusses the design of sedimentation velocity and sedimentation equilibrium experiments performed with a Beckman Optima XL-A or XL-I analytical ultracentrifuge. Optimal instrument settings and experimental design considerations are explained, and strategies for the analysis of experimental data with the UltraScan data analysis software package are presented. Special attention is paid to the strengths and weaknesses of the available detectors, and guidance is provided on how to extract maximum information from analytical ultracentrifugation experiments.

Original languageEnglish (US)
Pages (from-to)7.13.1-7.13.24
JournalCurrent Protocols in Protein Science
Issue numberSUPP.60
DOIs
StatePublished - 2010

Keywords

  • 2-dimensional spectrum analysis
  • UltraScan
  • absorbance optics
  • analytical ultracentrifugation
  • intensity measurements
  • sedimentation equilibrium
  • sedimentation velocity
  • solution studies

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry

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