Methods for Reducing Duration of Parabiosis in Development of Tolerance to Skin Homografts in Mice

Anatolio B. Cruz; Lloyd D. Maclean; J. Bradley Aust

doi:10.3181/00379727-112-28192

Methods for Reducing Duration of Parabiosis in Development of Tolerance to Skin Homografts in Mice

Anatolio B. Cruz, Lloyd D. Maclean, J. Bradley Aust

Research output: Contribution to journal › Article › peer-review

Abstract

Using C₃H and (Ax C₃H)F₁ mice in parabiosis, this study was undertaken to find means of reducing the duration of parabiosis necessary for development of immunologic tolerance. Parabiosis in the usual way maintained for 8–10 days did not result in development of tolerance. When talc powder was sprinkled and mechanical abrasion employed, 25% of the grafted C₃H mice became tolerant to the hybrid tissue. If talc powder was applied 6–7 days prior to parabiosis and the area of contact between parabiosis increased by creation of skin flaps, 85.7% of grafted C₃H mice became tolerant to (Ax C₃H)F₁ skin.

Original language	English (US)
Pages (from-to)	866-868
Number of pages	3
Journal	Proceedings of the Society for Experimental Biology and Medicine
Volume	112
Issue number	4
DOIs	https://doi.org/10.3181/00379727-112-28192
State	Published - Apr 1963
Externally published	Yes

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

Access to Document

10.3181/00379727-112-28192

Cite this

@article{64d24530bdf1425fbc634190cfe91240,

title = "Methods for Reducing Duration of Parabiosis in Development of Tolerance to Skin Homografts in Mice",

abstract = "Using C3H and (Ax C3H)F1 mice in parabiosis, this study was undertaken to find means of reducing the duration of parabiosis necessary for development of immunologic tolerance. Parabiosis in the usual way maintained for 8–10 days did not result in development of tolerance. When talc powder was sprinkled and mechanical abrasion employed, 25% of the grafted C3H mice became tolerant to the hybrid tissue. If talc powder was applied 6–7 days prior to parabiosis and the area of contact between parabiosis increased by creation of skin flaps, 85.7% of grafted C3H mice became tolerant to (Ax C3H)F1 skin.",

author = "Cruz, {Anatolio B.} and Maclean, {Lloyd D.} and Aust, {J. Bradley}",

year = "1963",

month = apr,

doi = "10.3181/00379727-112-28192",

language = "English (US)",

volume = "112",

pages = "866--868",

journal = "Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N. Y.)",

issn = "1535-3702",

publisher = "Society for Experimental Biology and Medicine",

number = "4",

}

TY - JOUR

T1 - Methods for Reducing Duration of Parabiosis in Development of Tolerance to Skin Homografts in Mice

AU - Cruz, Anatolio B.

AU - Maclean, Lloyd D.

AU - Aust, J. Bradley

PY - 1963/4

Y1 - 1963/4

N2 - Using C3H and (Ax C3H)F1 mice in parabiosis, this study was undertaken to find means of reducing the duration of parabiosis necessary for development of immunologic tolerance. Parabiosis in the usual way maintained for 8–10 days did not result in development of tolerance. When talc powder was sprinkled and mechanical abrasion employed, 25% of the grafted C3H mice became tolerant to the hybrid tissue. If talc powder was applied 6–7 days prior to parabiosis and the area of contact between parabiosis increased by creation of skin flaps, 85.7% of grafted C3H mice became tolerant to (Ax C3H)F1 skin.

AB - Using C3H and (Ax C3H)F1 mice in parabiosis, this study was undertaken to find means of reducing the duration of parabiosis necessary for development of immunologic tolerance. Parabiosis in the usual way maintained for 8–10 days did not result in development of tolerance. When talc powder was sprinkled and mechanical abrasion employed, 25% of the grafted C3H mice became tolerant to the hybrid tissue. If talc powder was applied 6–7 days prior to parabiosis and the area of contact between parabiosis increased by creation of skin flaps, 85.7% of grafted C3H mice became tolerant to (Ax C3H)F1 skin.

UR - http://www.scopus.com/inward/record.url?scp=84964164144&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84964164144&partnerID=8YFLogxK

U2 - 10.3181/00379727-112-28192

DO - 10.3181/00379727-112-28192

M3 - Article

AN - SCOPUS:84964164144

VL - 112

SP - 866

EP - 868

JO - Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N. Y.)

JF - Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N. Y.)

SN - 1535-3702

IS - 4

ER -

Methods for Reducing Duration of Parabiosis in Development of Tolerance to Skin Homografts in Mice

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this