TY - JOUR
T1 - Metallotyping of ketotic dairy cows reveals major alterations preceding, associating, and following the disease occurrence
AU - Zhang, Guanshi
AU - Dervishi, Elda
AU - Mandal, Rupasri
AU - Wishart, David S.
AU - Ametaj, Burim N.
N1 - Funding Information:
This research work was funded by Alberta Livestock and Meat Agency Ltd. (ALMA, Edmonton, AB, Canada), Genome Alberta (Calgary, AB, Canada), and Natural Sciences and Engineering Research Council of Canada (Ottawa, ON, Canada) with Dr. Burim N. Ametaj and Dr. David S. Wishart as Principal Investigators. We acknowledge the full or partial help of D. Hailemariam, S. A. Goldansaz, Q. Deng, and J. F. Odhiambo in collection of samples from cows. We are also grateful to the technical staff at Dairy Research and Technology Center, University of Alberta, for their help and care with the cows. The authors declare no conflict of interest.
Publisher Copyright:
© 2017, Springer Science+Business Media, LLC.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2017/8/1
Y1 - 2017/8/1
N2 - Introduction: Ketosis is a common metabolic disorder, which is characterized by elevated concentrations of ketone bodies or ketoacids in three body fluids including blood, urine, and milk. Two of the ketones including β-hydroxybutyric acid and acetoacetic acid are strong acids which at high concentrations trigger ketoacidosis influencing physiological functions of various tissues and organs. Objectives: The objectives of this study were to: (1) investigate mineral alterations in both serum and urine of preketotic, ketotic, and postketotic cows, (2) identify potential predictive and diagnostic mineral biomarkers for ketosis in serum and urine, and (3) better understand the role of minerals in the pathobiology of the disease. Methods: Inductively coupled plasma mass spectrometry metallotyping was performed in the serum and urine of six cases of ketosis and 20 healthy controls cows at −8 and −4 weeks prepartum, at disease diagnosis week, and at +4 and +8 weeks postpartum. Results: Data showed that concentrations of aluminum (Al), iron (Fe), manganese (Mn), and arsenic (As) were greater (P < 0.001) in the serum of preketotic, ketotic and postketotic cows at most of the tested time points. Moreover, boron (B) and Al as well as calcium (Ca), phosphorus (P), potassium (K), and magnesium (Mg) were found to be elevated in the urine of preketotic and postketotic cows (P < 0.001). Conclusions: It is concluded that alterations of mineral elements observed in the serum and urine of preketotic, ketotic, and postketotic cows might be related to the state of chronic acidosis in those cows. The mineral elements identified in both serum and urine can be used as biomarkers to early diagnose ketosis at its pre-subclinical state and develop preventive interventions in the future.
AB - Introduction: Ketosis is a common metabolic disorder, which is characterized by elevated concentrations of ketone bodies or ketoacids in three body fluids including blood, urine, and milk. Two of the ketones including β-hydroxybutyric acid and acetoacetic acid are strong acids which at high concentrations trigger ketoacidosis influencing physiological functions of various tissues and organs. Objectives: The objectives of this study were to: (1) investigate mineral alterations in both serum and urine of preketotic, ketotic, and postketotic cows, (2) identify potential predictive and diagnostic mineral biomarkers for ketosis in serum and urine, and (3) better understand the role of minerals in the pathobiology of the disease. Methods: Inductively coupled plasma mass spectrometry metallotyping was performed in the serum and urine of six cases of ketosis and 20 healthy controls cows at −8 and −4 weeks prepartum, at disease diagnosis week, and at +4 and +8 weeks postpartum. Results: Data showed that concentrations of aluminum (Al), iron (Fe), manganese (Mn), and arsenic (As) were greater (P < 0.001) in the serum of preketotic, ketotic and postketotic cows at most of the tested time points. Moreover, boron (B) and Al as well as calcium (Ca), phosphorus (P), potassium (K), and magnesium (Mg) were found to be elevated in the urine of preketotic and postketotic cows (P < 0.001). Conclusions: It is concluded that alterations of mineral elements observed in the serum and urine of preketotic, ketotic, and postketotic cows might be related to the state of chronic acidosis in those cows. The mineral elements identified in both serum and urine can be used as biomarkers to early diagnose ketosis at its pre-subclinical state and develop preventive interventions in the future.
KW - Dairy cow
KW - ICP-MS
KW - Ketosis
KW - Metallotyping
KW - Serum and urine
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U2 - 10.1007/s11306-017-1237-4
DO - 10.1007/s11306-017-1237-4
M3 - Article
AN - SCOPUS:85021742982
VL - 13
JO - Metabolomics
JF - Metabolomics
SN - 1573-3882
IS - 8
M1 - 97
ER -