Metabolism of endogenous surfactant in premature baboons and effect of prenatal corticosteroids

Jan Erik H. Bunt, Virgilio P. Carnielli, Steven R. Seidner, Machiko Ikegami, J. L.Darcos Wattimena, Pieter J.J. Sauer, Alan H. Jobe, Luc J.I. Zimmermann

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

We studied the synthesis of surfactant and the effect of prenatal betamethasone treatment in vivo in very preterm baboons. Ten pregnant baboons were randomized to receive either betamethasone (beta) or saline (control) 48 and 24 h before preterm delivery. The newborn baboons were intubated, treated with surfactant, and ventilated for 6 d. They received a 24-h infusion with the stable isotope [U-13C]glucose as precursor for the synthesis of palmitic acid in surfactant phosphatidylcholine (PC). Palmitic acid in surfactant PC became enriched 27 ± 2 h after the start of the isotope infusion and was maximally enriched at 100 ± 4 h. The fractional synthesis rate of PC palmitate in the beta group (1.5 ± 0.2%/d) was increased by 129% above control (0.7 ± 0.1%/d) (p < 0.02, Mann-Whitney U test). The absolute synthesis rate of PC in the beta group [1.6 ± 0.3 μmol/kg/d] was increased by 128% above controls [0.7 ± 0.2 μmol/kg/d] (p < 0.02). These data show that the synthesis of endogenous surfactant from plasma glucose as precursor is a slow process. It is shown, for the first time in vivo, that prenatal glucocorticosteroids stimulate the synthesis of surfactant PC in the very premature baboon.

Original languageEnglish (US)
Pages (from-to)1481-1485
Number of pages5
JournalAmerican Journal of Respiratory and Critical Care Medicine
Volume160
Issue number5 I
DOIs
StatePublished - 1999

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Critical Care and Intensive Care Medicine

Fingerprint Dive into the research topics of 'Metabolism of endogenous surfactant in premature baboons and effect of prenatal corticosteroids'. Together they form a unique fingerprint.

  • Cite this

    Bunt, J. E. H., Carnielli, V. P., Seidner, S. R., Ikegami, M., Wattimena, J. L. D., Sauer, P. J. J., Jobe, A. H., & Zimmermann, L. J. I. (1999). Metabolism of endogenous surfactant in premature baboons and effect of prenatal corticosteroids. American Journal of Respiratory and Critical Care Medicine, 160(5 I), 1481-1485. https://doi.org/10.1164/ajrccm.160.5.9808070