@article{da72a72734934b1a87e1f06720603099,
title = "Metabolic benefits of methionine restriction in adult mice do not require functional methionine sulfoxide reductase A (MsrA)",
abstract = "Methionine restriction (MR) extends lifespan and improves several markers of health in rodents. However, the proximate mechanisms of MR on these physiological benefits have not been fully elucidated. The essential amino acid methionine plays numerous biological roles and limiting its availability in the diet directly modulates methionine metabolism. There is growing evidence that redox regulation of methionine has regulatory control on some aspects of cellular function but interactions with MR remain largely unexplored. We tested the functional role of the ubiquitously expressed methionine repair enzyme methionine sulfoxide reductase A (MsrA) on the metabolic benefits of MR in mice. MsrA catalytically reduces both free and protein-bound oxidized methionine, thus playing a key role in its redox state. We tested the extent to which MsrA is required for metabolic effects of MR in adult mice using mice lacking MsrA. As expected, MR in control mice reduced body weight, altered body composition, and improved glucose metabolism. Interestingly, lack of MsrA did not impair the metabolic effects of MR on these outcomes. Moreover, females had blunted MR responses regardless of MsrA status compared to males. Overall, our data suggests that MsrA is not required for the metabolic benefits of MR in adult mice.",
author = "Thyne, {Kevin M.} and Salmon, {Adam B.}",
note = "Funding Information: We would also like to acknowledge the experimental assistance of Yuhong Liu and Jodie Cropper throughout this study, and Dr. Jonathan Dorigatti for experimental assistance and helpful discussions. Milliplex analyte panels were performed by Bioanalytics and Single-Cell (BASiC) Core at UT-Health San Antonio. Metabolic cage assessments were performed by the Integrated Physiology of Aging Core at the San Antonio Nathan Shock Center (P30 AG013319). This research was funded in part by R01 AG050797, R01 AG057431, T32 AG021890 and the San Antonio Area Foundation. ABS is partially supported by the Geriatric Research, Education and Clinical Center of the South Texas Veterans Health Care System. This material is the result of work supported with resources and the use of facilities at South Texas Veterans Health Care System, San Antonio, Texas. The contents do not represent the views of the U.S. Department of Veterans Affairs or the United States Government. Funding Information: We would also like to acknowledge the experimental assistance of Yuhong Liu and Jodie Cropper throughout this study, and Dr. Jonathan Dorigatti for experimental assistance and helpful discussions. Milliplex analyte panels were performed by Bioanalytics and Single-Cell (BASiC) Core at UT-Health San Antonio. Metabolic cage assessments were performed by the Integrated Physiology of Aging Core at the San Antonio Nathan Shock Center (P30 AG013319). This research was funded in part by R01 AG050797, R01 AG057431, T32 AG021890 and the San Antonio Area Foundation. ABS is partially supported by the Geriatric Research, Education and Clinical Center of the South Texas Veterans Health Care System. This material is the result of work supported with resources and the use of facilities at South Texas Veterans Health Care System, San Antonio, Texas. The contents do not represent the views of the U.S. Department of Veterans Affairs or the United States Government. Publisher Copyright: {\textcopyright} 2022, The Author(s).",
year = "2022",
month = dec,
doi = "10.1038/s41598-022-08978-4",
language = "English (US)",
volume = "12",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",
}