Mesothelium expression of integrins in vivo and in vitro

Craig A. Witz, Iris A. Montoya-Rodriguez, Dena M. Miller, Barbara G. Schneider, Robert S. Schenken

Research output: Contribution to journalArticlepeer-review

46 Scopus citations


OBJECTIVE: To characterize the expression of α subunits of integrin adhesion molecules in peritoneal tissue in vivo and in vitro. METHOD: Peritoneum from the anterior abdominal wall (n = 22) and the serosa of the posterior uterus (n = 11) was obtained from women of reproductive age without endometriosis who were undergoing surgery for benign conditions. Immunohistochemical studies were performed on serial sections of peritoneum from the anterior abdominal wall, the uterine serosa, mesothelial monolayer cultures, and peritoneum explants from the abdominal wall using monoclonal antibodies to α subunits of integrin adhesion molecules. Electron microscopy was performed to localize these adhesion molecules in the mesothelium. RESULTS: The mesothelial expression of α integrin subunits was identical in the anterior peritoneum and uterine serosa. In vivo the mesothelium strongly expressed α2 and α2 and variably expressed α6. In the monolayer cultures there was moderate/strong staining for α2, α3, and α5; there was minimal expression of αν. In the explants there was moderate/strong expression of α2, α3, α5, and α(v) α6 was variably expressed. The ultrastructure of the mesothelium was unique in the anterior peritoneum, uterine serosa, and the monolayer cultures. The integrin subunits were distributed throughout the cytoplasm, were expressed in the plasma membrane, and were present on the surface (ie, towards the peritoneal cavity) of the mesothelium. CONCLUSION: Integrins are expressed by the mesothelium of the peritoneum. The mesothelium expression of integrins in vivo differs from that of the mesothelium integrin expression in monolayer culture and explant culture.

Original languageEnglish (US)
Pages (from-to)87-93
Number of pages7
JournalJournal of the Society for Gynecologic Investigation
Issue number2
StatePublished - Mar 1998


  • Adhesion
  • Culture
  • Integrins
  • Mesothelium
  • Monolayer
  • Peritoneum

ASJC Scopus subject areas

  • Obstetrics and Gynecology


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