Mesangial cells express PDGF mRNAs and proliferate in response to PDGF

P. J. Shultz, P. E. DiCorleto, B. J. Silver, H. E. Abboud

Research output: Contribution to journalArticle

230 Scopus citations

Abstract

Platelet-derived growth factor (PDGF) is a potent mitogen for cells of mesenchymal origin and is released and/or synthesized by platelets, macrophages, endothelial cells, and rat mesangial cells. In the present investigation, we found that human glomerular mesangial cells in culture release a PDGF-like protein which competes for 125I-PDGF binding to human foreskin fibroblasts and is mitogenic for these fibroblasts. The competing and to a lesser extent the mitogenic activities present in the conditioned medium are partially recognized by an anti-PDGF antibody. Northern blot analysis of poly(A)+ RNA from human mesangial cells demonstrates the expression of both PDGF A- and B-chain mRNAs. PDGF also binds to mesangial cells in a specific manner and stimulates DNA synthesis and cell proliferation. These data suggest that a PDGF-like protein secreted by mesangial cells or released from platelets, monocytes, or endothelial cells during glomerular inflammation may function as an autocrine or a paracrine growth factor for these cells. The biological role of PDGF in mediating proliferative and other inflammatory events in the glomerulus remains to be identified.

Original languageEnglish (US)
Pages (from-to)F674-F684
JournalAmerican Journal of Physiology - Renal Fluid and Electrolyte Physiology
Volume255
Issue number4 (24/4)
DOIs
StatePublished - 1988
Externally publishedYes

ASJC Scopus subject areas

  • Physiology

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