MEPE Localization in the Craniofacial Complex and Function in Tooth Dentin Formation

Angela Gullard, Jelica Gluhak-Heinrich, Silvana Papagerakis, Philip Sohn, Aaron Unterbrink, Shuo Chen, Mary MacDougall

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Matrix extracellular phosphoglycoprotein (MEPE) is an extracellular matrix protein found in dental and skeletal tissues. Although information regarding the role of MEPE in bone and disorders of phosphate metabolism is emerging, the role of MEPE in dental tissues remains unclear. We performed RNA in situ hybridization and immunohistochemistry analyses to delineate the expression pattern of MEPE during embryonic and postnatal development in craniofacial mineralizing tissues. Mepe RNA expression was seen within teeth from cap through root formation in association with odontoblasts and cellular cementoblasts. More intense expression was seen in the alveolar bone within the osteoblasts and osteocytes. MEPE immunohistochemistry showed biphasic dentin staining in incisors and more intense staining in alveolar bone matrix and in forming cartilage. Analysis of Mepe null mouse molars showed overall mineralized tooth volume and density of enamel and dentin comparable with that of wild-type samples. However, Mepe-/- molars exhibited increased thickness of predentin, dentin, and enamel over controls and decreased gene expression of Enam, Bsp, Dmp1, Dspp, and Opn by RT-PCR. In vitro Mepe overexpression in odontoblasts led to significant reductions in Dspp reporter activity. These data suggest MEPE may be instrumental in craniofacial and dental matrix maturation, potentially functioning in the maintenance of non-mineralized matrix.

Original languageEnglish (US)
Pages (from-to)224-236
Number of pages13
JournalJournal of Histochemistry and Cytochemistry
Volume64
Issue number4
DOIs
StatePublished - 2016

Fingerprint

Dentin
Extracellular Matrix
Tooth
Odontoblasts
Dental Enamel
Immunohistochemistry
RNA
Staining and Labeling
Dental Cementum
Bone and Bones
Osteocytes
Bone Matrix
Extracellular Matrix Proteins
Incisor
Osteoblasts
Cartilage
Embryonic Development
In Situ Hybridization
Phosphates
Maintenance

Keywords

  • alveolar bone
  • dentin
  • immunohistochemistry
  • in situ hybridization
  • matrix mineralization
  • MEPE

ASJC Scopus subject areas

  • Anatomy
  • Histology

Cite this

Gullard, A., Gluhak-Heinrich, J., Papagerakis, S., Sohn, P., Unterbrink, A., Chen, S., & MacDougall, M. (2016). MEPE Localization in the Craniofacial Complex and Function in Tooth Dentin Formation. Journal of Histochemistry and Cytochemistry, 64(4), 224-236. https://doi.org/10.1369/0022155416635569

MEPE Localization in the Craniofacial Complex and Function in Tooth Dentin Formation. / Gullard, Angela; Gluhak-Heinrich, Jelica; Papagerakis, Silvana; Sohn, Philip; Unterbrink, Aaron; Chen, Shuo; MacDougall, Mary.

In: Journal of Histochemistry and Cytochemistry, Vol. 64, No. 4, 2016, p. 224-236.

Research output: Contribution to journalArticle

Gullard, A, Gluhak-Heinrich, J, Papagerakis, S, Sohn, P, Unterbrink, A, Chen, S & MacDougall, M 2016, 'MEPE Localization in the Craniofacial Complex and Function in Tooth Dentin Formation', Journal of Histochemistry and Cytochemistry, vol. 64, no. 4, pp. 224-236. https://doi.org/10.1369/0022155416635569
Gullard, Angela ; Gluhak-Heinrich, Jelica ; Papagerakis, Silvana ; Sohn, Philip ; Unterbrink, Aaron ; Chen, Shuo ; MacDougall, Mary. / MEPE Localization in the Craniofacial Complex and Function in Tooth Dentin Formation. In: Journal of Histochemistry and Cytochemistry. 2016 ; Vol. 64, No. 4. pp. 224-236.
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