Meox2Cre-mediated disruption of CSF-1 leads to osteopetrosis and osteocyte defects

Stephen E. Harris, Mary MacDougall, Diane Horn, Kathleen Woodruff, Stephanie N. Zimmer, Vivienne I. Rebel, Roberto Fajardo, Jian Q. Feng, Jelica Gluhak-Heinrich, Marie A. Harris, Sherry Abboud Werner

Research output: Contribution to journalArticle

45 Scopus citations

Abstract

CSF-1, a key regulator of mononuclear phagocyte production, is highly expressed in the skeleton by osteoblasts/osteocytes and in a number of nonskeletal tissues such as uterus, kidney and brain. The spontaneous mutant op/op mouse has been the conventional model of CSF-1 deficiency and exhibits a pleiotropic phenotype characterized by osteopetrosis, and defects in hematopoiesis, fertility and neural function. Studies to further delineate the biologic effect of CSF-1 within various tissues have been hampered by the lack of suitable models. To address this issue, we generated CSF-1 floxed/floxed mice and demonstrate that Cre-mediated recombination using Meox2Cre, a Cre line expressed in epiblast during early embryogenesis, results in mice with ubiquitous CSF-1 deficiency (CSF-1KO). Homozygous CSF-1KO mice lacked CSF-1 in all tissues and displayed, in part, a similar phenotype to op/op mice that included: failure of tooth eruption, osteopetrosis, reduced macrophage densities in reproductive and other organs and altered hematopoiesis with decreased marrow cellularity, circulating monocytes and B cell lymphopoiesis. In contrast to op/op mice, CSF-1KO mice showed elevated circulating and splenic T cells. A striking feature in CSF-1KO mice was defective osteocyte maturation, bone mineralization and osteocyte-lacunar system that was associated with reduced dentin matrix protein 1 (DMP1) expression in osteocytes. CSF-1KO mice also showed a dramatic reduction in osteomacs along the endosteal surface that may have contributed to the hematopoietic and cortical bone defects. Thus, our findings show that ubiquitous CSF-1 gene deletion using a Cre-based system recapitulates the expected osteopetrotic phenotype. Moreover, results point to a novel link between CSF-1 and osteocyte survival/function that is essential for maintaining bone mass and strength during skeletal development.

Original languageEnglish (US)
Pages (from-to)42-53
Number of pages12
JournalBone
Volume50
Issue number1
DOIs
StatePublished - Jan 2012

Keywords

  • Colony stimulating factor-1 (CSF-1)
  • Dentin matrix protein 1 (DMP1)
  • Knockout mouse
  • Osteoblasts
  • Osteocytes

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Histology

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    Harris, S. E., MacDougall, M., Horn, D., Woodruff, K., Zimmer, S. N., Rebel, V. I., Fajardo, R., Feng, J. Q., Gluhak-Heinrich, J., Harris, M. A., & Abboud Werner, S. (2012). Meox2Cre-mediated disruption of CSF-1 leads to osteopetrosis and osteocyte defects. Bone, 50(1), 42-53. https://doi.org/10.1016/j.bone.2011.09.038