Memory deficit associated with increased brain proinflammatory cytokine levels and neurodegeneration in acute ischemic stroke

Bruno Silva, Larissa Sousa, Aline Miranda, Anilton Vasconcelos, Helton Reis, Lucíola Barcelos, Rosa Arantes, Antonio Teixeira, Milene Alvarenga Rachid

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

The present study aimed to investigate behavioral changes and neuroinflammatory process following left unilateral common carotid artery occlusion (UCCAO), a model of cerebral ischemia. Post-ischemic behavioral changes following 15 min UCCAO were recorded 24 hours after reperfusion. The novel object recognition task was used to assess learning and memory. After behavioral test, brains from sham and ischemic mice were removed and processed to evaluate central nervous system pathology by TTC and H&E techniques as well as inflammatory mediators by ELISA. UCCAO promoted long-term memory impairment after reperfusion. Infarct areas were observed in the cerebrum by TTC stain. Moreover, the histopathological analysis revealed cerebral necrotic cavities surrounded by ischemic neurons and hippocampal neurodegeneration. In parallel with memory dysfunction, brain levels of TNF-a, IL-1b and CXCL1 were increased post ischemia compared with sham-operated group. These findings suggest an involvement of central nervous system inflammatory mediators and brain damage in cognitive impairment following unilateral acute ischemia.

Original languageEnglish (US)
Pages (from-to)655-659
Number of pages5
JournalArquivos de neuro-psiquiatria
Volume73
Issue number8
DOIs
StatePublished - Jul 31 2015
Externally publishedYes

Keywords

  • Brain
  • Cytokines
  • Inflammation
  • Ischemia
  • Memory

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology
  • Biological Psychiatry

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