Membranous nephropathy and cerebellar degeneration with anti-GAD antibodies in type 2 diabetes mellitus

Franco Folli, Giuseppe Daniele, Sherry Werner, Subash Kamath, Jose Velez Munera, Hanna Abboud, Paolo Fanti

Research output: Contribution to journalArticle

Abstract

Aims: To study the potential pathogenic significance of the coexistence of membranous nephropathy, cerebellar degeneration and anti-glutamic acid decarboxylase (GAD) autoantibodies in patients with diabetes.

Methods: We performed a direct immunocytochemistry on human kidney slides, electron microscopy on human kidney biopsy, direct immunofluorescence on human kidney biopsy. Baboon and rat kidney cell lines were fractionated and subjected to western blotting with antibodies to GAD.

Results: In this patient we demonstrate the presence of autoantibodies to GAD, which is highly enriched in podocytes plasma membrane and tubular cells of the kidney as well as sub-endothelial IgG and complement C3 deposits in the glomerular basement membrane (GBM).

Conclusions: We hypothesize the existence in this patient of a common autoimmune pathogenic mechanism with GAD as the autoantigenic determinant, underlying cerebellar degeneration and membranous nephropathy.

Original languageEnglish (US)
JournalActa Diabetologica
DOIs
StateAccepted/In press - Mar 10 2015

Fingerprint

Membranous Glomerulonephritis
Glutamate Decarboxylase
Type 2 Diabetes Mellitus
Kidney
Antibodies
Autoantibodies
Biopsy
Podocytes
Direct Fluorescent Antibody Technique
Glomerular Basement Membrane
Complement C3
Papio
Electron Microscopy
Immunoglobulin G
Western Blotting
Immunohistochemistry
Cell Membrane
Cell Line

Keywords

  • Anti-glutamic acid decarboxylase (GAD) autoantibodies
  • Cerebellar degeneration
  • Membranous nephropathy
  • Type 2 diabetes mellitus

ASJC Scopus subject areas

  • Endocrinology
  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Folli, F., Daniele, G., Werner, S., Kamath, S., Munera, J. V., Abboud, H., & Fanti, P. (Accepted/In press). Membranous nephropathy and cerebellar degeneration with anti-GAD antibodies in type 2 diabetes mellitus. Acta Diabetologica. https://doi.org/10.1007/s00592-015-0728-x

Membranous nephropathy and cerebellar degeneration with anti-GAD antibodies in type 2 diabetes mellitus. / Folli, Franco; Daniele, Giuseppe; Werner, Sherry; Kamath, Subash; Munera, Jose Velez; Abboud, Hanna; Fanti, Paolo.

In: Acta Diabetologica, 10.03.2015.

Research output: Contribution to journalArticle

Folli, Franco ; Daniele, Giuseppe ; Werner, Sherry ; Kamath, Subash ; Munera, Jose Velez ; Abboud, Hanna ; Fanti, Paolo. / Membranous nephropathy and cerebellar degeneration with anti-GAD antibodies in type 2 diabetes mellitus. In: Acta Diabetologica. 2015.
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