Membranous nephropathy and cerebellar degeneration with anti-GAD antibodies in type 2 diabetes mellitus

Franco Folli; Giuseppe Daniele; Sherry Werner; Subash Kamath; Jose Velez Munera; Hanna Abboud; Paolo Fanti

doi:10.1007/s00592-015-0728-x

Membranous nephropathy and cerebellar degeneration with anti-GAD antibodies in type 2 diabetes mellitus

Franco Folli, Giuseppe Daniele, Sherry Werner, Subash Kamath, Jose Velez Munera, Hanna Abboud, Paolo Fanti

Research output: Contribution to journal › Article › peer-review

Abstract

Aims: To study the potential pathogenic significance of the coexistence of membranous nephropathy, cerebellar degeneration and anti-glutamic acid decarboxylase (GAD) autoantibodies in patients with diabetes.

Methods: We performed a direct immunocytochemistry on human kidney slides, electron microscopy on human kidney biopsy, direct immunofluorescence on human kidney biopsy. Baboon and rat kidney cell lines were fractionated and subjected to western blotting with antibodies to GAD.

Results: In this patient we demonstrate the presence of autoantibodies to GAD, which is highly enriched in podocytes plasma membrane and tubular cells of the kidney as well as sub-endothelial IgG and complement C3 deposits in the glomerular basement membrane (GBM).

Conclusions: We hypothesize the existence in this patient of a common autoimmune pathogenic mechanism with GAD as the autoantigenic determinant, underlying cerebellar degeneration and membranous nephropathy.

Original language	English (US)
Journal	Acta Diabetologica
DOIs	https://doi.org/10.1007/s00592-015-0728-x
State	Accepted/In press - Mar 10 2015

Keywords

Anti-glutamic acid decarboxylase (GAD) autoantibodies
Cerebellar degeneration
Membranous nephropathy
Type 2 diabetes mellitus

ASJC Scopus subject areas

Endocrinology
Internal Medicine
Endocrinology, Diabetes and Metabolism

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title = "Membranous nephropathy and cerebellar degeneration with anti-GAD antibodies in type 2 diabetes mellitus",

abstract = "Aims: To study the potential pathogenic significance of the coexistence of membranous nephropathy, cerebellar degeneration and anti-glutamic acid decarboxylase (GAD) autoantibodies in patients with diabetes.Methods: We performed a direct immunocytochemistry on human kidney slides, electron microscopy on human kidney biopsy, direct immunofluorescence on human kidney biopsy. Baboon and rat kidney cell lines were fractionated and subjected to western blotting with antibodies to GAD.Results: In this patient we demonstrate the presence of autoantibodies to GAD, which is highly enriched in podocytes plasma membrane and tubular cells of the kidney as well as sub-endothelial IgG and complement C3 deposits in the glomerular basement membrane (GBM).Conclusions: We hypothesize the existence in this patient of a common autoimmune pathogenic mechanism with GAD as the autoantigenic determinant, underlying cerebellar degeneration and membranous nephropathy.",

keywords = "Anti-glutamic acid decarboxylase (GAD) autoantibodies, Cerebellar degeneration, Membranous nephropathy, Type 2 diabetes mellitus",

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T1 - Membranous nephropathy and cerebellar degeneration with anti-GAD antibodies in type 2 diabetes mellitus

AU - Folli, Franco

AU - Daniele, Giuseppe

AU - Werner, Sherry

AU - Kamath, Subash

AU - Munera, Jose Velez

AU - Abboud, Hanna

AU - Fanti, Paolo

PY - 2015/3/10

Y1 - 2015/3/10

N2 - Aims: To study the potential pathogenic significance of the coexistence of membranous nephropathy, cerebellar degeneration and anti-glutamic acid decarboxylase (GAD) autoantibodies in patients with diabetes.Methods: We performed a direct immunocytochemistry on human kidney slides, electron microscopy on human kidney biopsy, direct immunofluorescence on human kidney biopsy. Baboon and rat kidney cell lines were fractionated and subjected to western blotting with antibodies to GAD.Results: In this patient we demonstrate the presence of autoantibodies to GAD, which is highly enriched in podocytes plasma membrane and tubular cells of the kidney as well as sub-endothelial IgG and complement C3 deposits in the glomerular basement membrane (GBM).Conclusions: We hypothesize the existence in this patient of a common autoimmune pathogenic mechanism with GAD as the autoantigenic determinant, underlying cerebellar degeneration and membranous nephropathy.

AB - Aims: To study the potential pathogenic significance of the coexistence of membranous nephropathy, cerebellar degeneration and anti-glutamic acid decarboxylase (GAD) autoantibodies in patients with diabetes.Methods: We performed a direct immunocytochemistry on human kidney slides, electron microscopy on human kidney biopsy, direct immunofluorescence on human kidney biopsy. Baboon and rat kidney cell lines were fractionated and subjected to western blotting with antibodies to GAD.Results: In this patient we demonstrate the presence of autoantibodies to GAD, which is highly enriched in podocytes plasma membrane and tubular cells of the kidney as well as sub-endothelial IgG and complement C3 deposits in the glomerular basement membrane (GBM).Conclusions: We hypothesize the existence in this patient of a common autoimmune pathogenic mechanism with GAD as the autoantigenic determinant, underlying cerebellar degeneration and membranous nephropathy.

KW - Anti-glutamic acid decarboxylase (GAD) autoantibodies

KW - Cerebellar degeneration

KW - Membranous nephropathy

KW - Type 2 diabetes mellitus

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