Abstract
Aims: To study the potential pathogenic significance of the coexistence of membranous nephropathy, cerebellar degeneration and anti-glutamic acid decarboxylase (GAD) autoantibodies in patients with diabetes.
Methods: We performed a direct immunocytochemistry on human kidney slides, electron microscopy on human kidney biopsy, direct immunofluorescence on human kidney biopsy. Baboon and rat kidney cell lines were fractionated and subjected to western blotting with antibodies to GAD.
Results: In this patient we demonstrate the presence of autoantibodies to GAD, which is highly enriched in podocytes plasma membrane and tubular cells of the kidney as well as sub-endothelial IgG and complement C3 deposits in the glomerular basement membrane (GBM).
Conclusions: We hypothesize the existence in this patient of a common autoimmune pathogenic mechanism with GAD as the autoantigenic determinant, underlying cerebellar degeneration and membranous nephropathy.
| Original language | English (US) |
|---|---|
| Journal | Acta Diabetologica |
| DOIs | |
| State | Accepted/In press - Mar 10 2015 |
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Keywords
- Anti-glutamic acid decarboxylase (GAD) autoantibodies
- Cerebellar degeneration
- Membranous nephropathy
- Type 2 diabetes mellitus
ASJC Scopus subject areas
- Endocrinology
- Internal Medicine
- Endocrinology, Diabetes and Metabolism
Cite this
Membranous nephropathy and cerebellar degeneration with anti-GAD antibodies in type 2 diabetes mellitus. / Folli, Franco; Daniele, Giuseppe; Werner, Sherry; Kamath, Subash; Munera, Jose Velez; Abboud, Hanna; Fanti, Paolo.
In: Acta Diabetologica, 10.03.2015.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Membranous nephropathy and cerebellar degeneration with anti-GAD antibodies in type 2 diabetes mellitus
AU - Folli, Franco
AU - Daniele, Giuseppe
AU - Werner, Sherry
AU - Kamath, Subash
AU - Munera, Jose Velez
AU - Abboud, Hanna
AU - Fanti, Paolo
PY - 2015/3/10
Y1 - 2015/3/10
N2 - Aims: To study the potential pathogenic significance of the coexistence of membranous nephropathy, cerebellar degeneration and anti-glutamic acid decarboxylase (GAD) autoantibodies in patients with diabetes.Methods: We performed a direct immunocytochemistry on human kidney slides, electron microscopy on human kidney biopsy, direct immunofluorescence on human kidney biopsy. Baboon and rat kidney cell lines were fractionated and subjected to western blotting with antibodies to GAD.Results: In this patient we demonstrate the presence of autoantibodies to GAD, which is highly enriched in podocytes plasma membrane and tubular cells of the kidney as well as sub-endothelial IgG and complement C3 deposits in the glomerular basement membrane (GBM).Conclusions: We hypothesize the existence in this patient of a common autoimmune pathogenic mechanism with GAD as the autoantigenic determinant, underlying cerebellar degeneration and membranous nephropathy.
AB - Aims: To study the potential pathogenic significance of the coexistence of membranous nephropathy, cerebellar degeneration and anti-glutamic acid decarboxylase (GAD) autoantibodies in patients with diabetes.Methods: We performed a direct immunocytochemistry on human kidney slides, electron microscopy on human kidney biopsy, direct immunofluorescence on human kidney biopsy. Baboon and rat kidney cell lines were fractionated and subjected to western blotting with antibodies to GAD.Results: In this patient we demonstrate the presence of autoantibodies to GAD, which is highly enriched in podocytes plasma membrane and tubular cells of the kidney as well as sub-endothelial IgG and complement C3 deposits in the glomerular basement membrane (GBM).Conclusions: We hypothesize the existence in this patient of a common autoimmune pathogenic mechanism with GAD as the autoantigenic determinant, underlying cerebellar degeneration and membranous nephropathy.
KW - Anti-glutamic acid decarboxylase (GAD) autoantibodies
KW - Cerebellar degeneration
KW - Membranous nephropathy
KW - Type 2 diabetes mellitus
UR - http://www.scopus.com/inward/record.url?scp=84924368120&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84924368120&partnerID=8YFLogxK
U2 - 10.1007/s00592-015-0728-x
DO - 10.1007/s00592-015-0728-x
M3 - Article
C2 - 25753172
AN - SCOPUS:84941937486
JO - Acta Diabetologica
JF - Acta Diabetologica
SN - 0940-5429
ER -