Membrane phospholipid composition may contribute to exceptional longevity of the naked mole-rat (Heterocephalus glaber): A comparative study using shotgun lipidomics

Todd W. Mitchell, Rochelle Buffenstein, A. J. Hulbert

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

Phospholipids containing highly polyunsaturated fatty acids are particularly prone to peroxidation and membrane composition may therefore influence longevity. Phospholipid molecules, in particular those containing docosahexaenoic acid (DHA), from the skeletal muscle, heart, liver and liver mitochondria were identified and quantified using mass-spectrometry shotgun lipidomics in two similar-sized rodents that show a ∼9-fold difference in maximum lifespan. The naked mole rat is the longest-living rodent known with a maximum lifespan of >28 years. Total phospholipid distribution is similar in tissues of both species; DHA is only found in phosphatidylcholines (PC), phosphatidylethanolamines (PE) and phosphatidylserines (PS), and DHA is relatively more concentrated in PE than PC. Naked mole-rats have fewer molecular species of both PC and PE than do mice. DHA-containing phospholipids represent 27-57% of all phospholipids in mice but only 2-6% in naked mole-rats. Furthermore, while mice have small amounts of di-polyunsaturated PC and PE, these are lacking in naked mole-rats. Vinyl ether-linked phospholipids (plasmalogens) are higher in naked mole-rat tissues than in mice. The lower level of DHA-containing phospholipids suggests a lower susceptibility to peroxidative damage in membranes of naked mole-rats compared to mice. Whereas the high level of plasmalogens might enhance membrane antioxidant protection in naked mole-rats compared to mice. Both characteristics possibly contribute to the exceptional longevity of naked mole-rats and may indicate a special role for peroxisomes in this extended longevity.

Original languageEnglish (US)
Pages (from-to)1053-1062
Number of pages10
JournalExperimental Gerontology
Volume42
Issue number11
DOIs
StatePublished - Nov 2007

Fingerprint

Mole Rats
Firearms
Rats
Phospholipids
Docosahexaenoic Acids
Phosphatidylethanolamines
Membranes
Chemical analysis
Phosphatidylcholines
Plasmalogens
Liver
Rodentia
Phospholipid Ethers
Tissue
Muscle Mitochondrion
Heart Mitochondria
Mitochondria
Peroxisomes
Liver Mitochondrion
Phosphatidylserines

Keywords

  • Docosahexaenoic acid
  • Lipid peroxidation
  • Longevity
  • Maximum lifespan
  • Membrane lipids
  • Naked mole-rat
  • Polyunsaturated fatty acids

ASJC Scopus subject areas

  • Aging
  • Medicine(all)

Cite this

Membrane phospholipid composition may contribute to exceptional longevity of the naked mole-rat (Heterocephalus glaber) : A comparative study using shotgun lipidomics. / Mitchell, Todd W.; Buffenstein, Rochelle; Hulbert, A. J.

In: Experimental Gerontology, Vol. 42, No. 11, 11.2007, p. 1053-1062.

Research output: Contribution to journalArticle

@article{54cd6189c8a34c15b633e4b31bd40093,
title = "Membrane phospholipid composition may contribute to exceptional longevity of the naked mole-rat (Heterocephalus glaber): A comparative study using shotgun lipidomics",
abstract = "Phospholipids containing highly polyunsaturated fatty acids are particularly prone to peroxidation and membrane composition may therefore influence longevity. Phospholipid molecules, in particular those containing docosahexaenoic acid (DHA), from the skeletal muscle, heart, liver and liver mitochondria were identified and quantified using mass-spectrometry shotgun lipidomics in two similar-sized rodents that show a ∼9-fold difference in maximum lifespan. The naked mole rat is the longest-living rodent known with a maximum lifespan of >28 years. Total phospholipid distribution is similar in tissues of both species; DHA is only found in phosphatidylcholines (PC), phosphatidylethanolamines (PE) and phosphatidylserines (PS), and DHA is relatively more concentrated in PE than PC. Naked mole-rats have fewer molecular species of both PC and PE than do mice. DHA-containing phospholipids represent 27-57{\%} of all phospholipids in mice but only 2-6{\%} in naked mole-rats. Furthermore, while mice have small amounts of di-polyunsaturated PC and PE, these are lacking in naked mole-rats. Vinyl ether-linked phospholipids (plasmalogens) are higher in naked mole-rat tissues than in mice. The lower level of DHA-containing phospholipids suggests a lower susceptibility to peroxidative damage in membranes of naked mole-rats compared to mice. Whereas the high level of plasmalogens might enhance membrane antioxidant protection in naked mole-rats compared to mice. Both characteristics possibly contribute to the exceptional longevity of naked mole-rats and may indicate a special role for peroxisomes in this extended longevity.",
keywords = "Docosahexaenoic acid, Lipid peroxidation, Longevity, Maximum lifespan, Membrane lipids, Naked mole-rat, Polyunsaturated fatty acids",
author = "Mitchell, {Todd W.} and Rochelle Buffenstein and Hulbert, {A. J.}",
year = "2007",
month = "11",
doi = "10.1016/j.exger.2007.09.004",
language = "English (US)",
volume = "42",
pages = "1053--1062",
journal = "Experimental Gerontology",
issn = "0531-5565",
publisher = "Elsevier Inc.",
number = "11",

}

TY - JOUR

T1 - Membrane phospholipid composition may contribute to exceptional longevity of the naked mole-rat (Heterocephalus glaber)

T2 - A comparative study using shotgun lipidomics

AU - Mitchell, Todd W.

AU - Buffenstein, Rochelle

AU - Hulbert, A. J.

PY - 2007/11

Y1 - 2007/11

N2 - Phospholipids containing highly polyunsaturated fatty acids are particularly prone to peroxidation and membrane composition may therefore influence longevity. Phospholipid molecules, in particular those containing docosahexaenoic acid (DHA), from the skeletal muscle, heart, liver and liver mitochondria were identified and quantified using mass-spectrometry shotgun lipidomics in two similar-sized rodents that show a ∼9-fold difference in maximum lifespan. The naked mole rat is the longest-living rodent known with a maximum lifespan of >28 years. Total phospholipid distribution is similar in tissues of both species; DHA is only found in phosphatidylcholines (PC), phosphatidylethanolamines (PE) and phosphatidylserines (PS), and DHA is relatively more concentrated in PE than PC. Naked mole-rats have fewer molecular species of both PC and PE than do mice. DHA-containing phospholipids represent 27-57% of all phospholipids in mice but only 2-6% in naked mole-rats. Furthermore, while mice have small amounts of di-polyunsaturated PC and PE, these are lacking in naked mole-rats. Vinyl ether-linked phospholipids (plasmalogens) are higher in naked mole-rat tissues than in mice. The lower level of DHA-containing phospholipids suggests a lower susceptibility to peroxidative damage in membranes of naked mole-rats compared to mice. Whereas the high level of plasmalogens might enhance membrane antioxidant protection in naked mole-rats compared to mice. Both characteristics possibly contribute to the exceptional longevity of naked mole-rats and may indicate a special role for peroxisomes in this extended longevity.

AB - Phospholipids containing highly polyunsaturated fatty acids are particularly prone to peroxidation and membrane composition may therefore influence longevity. Phospholipid molecules, in particular those containing docosahexaenoic acid (DHA), from the skeletal muscle, heart, liver and liver mitochondria were identified and quantified using mass-spectrometry shotgun lipidomics in two similar-sized rodents that show a ∼9-fold difference in maximum lifespan. The naked mole rat is the longest-living rodent known with a maximum lifespan of >28 years. Total phospholipid distribution is similar in tissues of both species; DHA is only found in phosphatidylcholines (PC), phosphatidylethanolamines (PE) and phosphatidylserines (PS), and DHA is relatively more concentrated in PE than PC. Naked mole-rats have fewer molecular species of both PC and PE than do mice. DHA-containing phospholipids represent 27-57% of all phospholipids in mice but only 2-6% in naked mole-rats. Furthermore, while mice have small amounts of di-polyunsaturated PC and PE, these are lacking in naked mole-rats. Vinyl ether-linked phospholipids (plasmalogens) are higher in naked mole-rat tissues than in mice. The lower level of DHA-containing phospholipids suggests a lower susceptibility to peroxidative damage in membranes of naked mole-rats compared to mice. Whereas the high level of plasmalogens might enhance membrane antioxidant protection in naked mole-rats compared to mice. Both characteristics possibly contribute to the exceptional longevity of naked mole-rats and may indicate a special role for peroxisomes in this extended longevity.

KW - Docosahexaenoic acid

KW - Lipid peroxidation

KW - Longevity

KW - Maximum lifespan

KW - Membrane lipids

KW - Naked mole-rat

KW - Polyunsaturated fatty acids

UR - http://www.scopus.com/inward/record.url?scp=35448999303&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=35448999303&partnerID=8YFLogxK

U2 - 10.1016/j.exger.2007.09.004

DO - 10.1016/j.exger.2007.09.004

M3 - Article

C2 - 18029129

AN - SCOPUS:35448999303

VL - 42

SP - 1053

EP - 1062

JO - Experimental Gerontology

JF - Experimental Gerontology

SN - 0531-5565

IS - 11

ER -