Melatonin

The watchdog of villous trophoblast homeostasis against hypoxia/reoxygenation-induced oxidative stress and apoptosis

Dave Lanoix, Andrée Anne Lacasse, Russel J Reiter, Cathy Vaillancourt

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Human placenta produces melatonin and expresses its receptors. We propose that melatonin, an antioxidant, protects the human placenta against hypoxia/reoxygenation (H/R)-induced damage. Primary term villous cytotrophoblasts were cultured under normoxia (8% O2) with or without 1mM melatonin for 72h to induce differentiation into the syncytiotrophoblast. The cells were then cultured for an additional 22h under normoxia or subjected to hypoxia (0.5% O2) for 4h followed by 18h reoxygenation (8% O2) with or without melatonin. H/R induced oxidative stress, which activated the Bax/Bcl-2 mitochondrial apoptosis pathway and the downstream fragmentation of DNA. Villous trophoblast treatment with melatonin reversed all the negative effects induced by H/R to normoxic levels. This study shows that melatonin protects the villous trophoblast against H/R-induced oxidative stress and apoptosis and suggests a potential preventive and therapeutic use of this indolamine in pregnancy complications characterized by syncytiotrophoblast survival alteration.

Original languageEnglish (US)
Pages (from-to)35-45
Number of pages11
JournalMolecular and Cellular Endocrinology
Volume381
Issue number1-2
DOIs
StatePublished - Dec 5 2013

Fingerprint

Oxidative stress
Trophoblasts
Melatonin
Oxidative Stress
Homeostasis
Apoptosis
Placenta
Pregnancy Complications
Therapeutic Uses
DNA Fragmentation
Hypoxia
Antioxidants
Survival
DNA

Keywords

  • Antioxidant
  • Mitochondrial apoptosis
  • Reactive oxygen species
  • Villous trophoblast

ASJC Scopus subject areas

  • Endocrinology
  • Molecular Biology
  • Biochemistry

Cite this

Melatonin : The watchdog of villous trophoblast homeostasis against hypoxia/reoxygenation-induced oxidative stress and apoptosis. / Lanoix, Dave; Lacasse, Andrée Anne; Reiter, Russel J; Vaillancourt, Cathy.

In: Molecular and Cellular Endocrinology, Vol. 381, No. 1-2, 05.12.2013, p. 35-45.

Research output: Contribution to journalArticle

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