Melatonin reduces protein and lipid oxidative damage induced by homocysteine in rat brain homogenates

Santiago Ortega-Gutiérrez, Lorena Fuentes-Broto, Joaquín J. García, Marta López-Vicente, Enrique Martínez-Ballarín, Francisco Javier Miana-Mena, Sergio Millán-Plano, Russel J. Reiter

Research output: Contribution to journalArticlepeer-review

26 Scopus citations


Numerous data indicate that hyperhomocysteinemia is a risk factor for cardio- and cerebrovascular diseases. At least in part, homocysteine (HCY) impairs cerebrovascular function because it generates large numbers of free radicals. Since melatonin is a well-known antioxidant, which reduces oxidative stress and decreases HCY concentrations in plasma, the aim of this study was to investigate the effect of melatonin in preventing HCY-induced protein and lipid oxidation in rat brain homogenates. Brain homogenates were obtained from Sprague-Dawley rats and were incubated with or without HCY (0.01-5 mM) or melatonin (0.01-3 mM). Carbonyl content of proteins, and malondialdehyde (MDA) and 4-hydroxyalkenals (4-HDA) concentrations in the brain homogenates were used as an index of protein and lipid oxidation, respectively. Under the experimental conditions used, the addition of HCY (0.01-5 mM) to the homogenates enhanced carbonyl protein and MDA+4-HDA formation. Melatonin reduced, in a concentration-dependent manner, protein and lipid oxidation due to HCY in the brain homogenates. These data suggest that preserving proteins from oxidative insults is an additional mechanism by which melatonin may act as an agent in potentially decreasing cardiovascular and cerebrovascular diseases related to hyperhomocysteinemia.

Original languageEnglish (US)
Pages (from-to)729-735
Number of pages7
JournalJournal of Cellular Biochemistry
Issue number3
StatePublished - Oct 15 2007
Externally publishedYes


  • Antioxidant therapy
  • Brain
  • Homocysteine
  • Lipid peroxidation
  • Melatonin
  • Protein oxidation

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry
  • Cell Biology


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