Melatonin reduces kainate-induced lipid peroxidation in homogenates of different brain regions

Daniela Melchiorri, Russel J. Reiter, Ewa Sewerynek, Li Dun Chen, Giuseppe Nisticó

Research output: Contribution to journalArticlepeer-review

162 Scopus citations

Abstract

Melatonin protection against in vitro kainic acid-induced oxidative damage in homogenates from different rat brain regions is shown. Brain-disrupted cell homogenates from cerebral cortex, cerebellum, hippocampus, hypothalamus, and corpus striatum were incubated with kainate (11.7 mM) with or without different concentrations of melatonin (0.1-4. mM). The concentration of malonaldehyde and 4-hydroxyalkenals was measured as an index of lipid peroxidation. Wistar and Sprague-Dawley rats were used. When administered with kainate, melatonin markedly reduced lipid peroxidation in every brain region of both rat strains. The reduction in lipid peroxidation by melatonin was concentration-dependent and varied from 10% to 100%. The protection conferred by melatonin is likely due, at least in part, to its newly discovered, free radical scavenging ability.

Original languageEnglish (US)
Pages (from-to)1205-1210
Number of pages6
JournalFASEB Journal
Volume9
Issue number12
DOIs
StatePublished - 1995

Keywords

  • excitatory neurotransmitter
  • free radicals
  • malonaldehyde
  • neuroprotection
  • oxidative stress

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

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