Melatonin reduces kainate-induced lipid peroxidation in homogenates of different brain regions

D. Melchiorri, R. J. Reiter, E. Sewerynek, Dun Chen Li Dun Chen, G. Nistico

Research output: Contribution to journalArticle

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Abstract

Melatonin protection against in vitro kainic acid-induced oxidative damage in homogenates from different rat brain regions is shown. Brain-disrupted cell homogenates from cerebral cortex, cerebellum, hippocampus, hypothalamus, and corpus striatum were incubated with kainate (11.7 mM) with or without different concentrations of melatonin (0.1-4. mM). The concentration of malonaldehyde and 4-hydroxyalkenals was measured as an index of lipid peroxidation. Wistar and Sprague-Dawley rats were used. When administered with kainate, melatonin markedly reduced lipid peroxidation in every brain region of both rat strains. The reduction in lipid peroxidation by melatonin was concentration-dependent and varied from 10% to 100%. The protection conferred by melatonin is likely due, at least in part, to its newly discovered, free radical scavenging ability.

Original languageEnglish (US)
Pages (from-to)1205-1210
Number of pages6
JournalFASEB Journal
Volume9
Issue number12
StatePublished - Jan 1 1995

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Keywords

  • excitatory neurotransmitter
  • free radicals
  • malonaldehyde
  • neuroprotection
  • oxidative stress

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Melchiorri, D., Reiter, R. J., Sewerynek, E., Li Dun Chen, D. C., & Nistico, G. (1995). Melatonin reduces kainate-induced lipid peroxidation in homogenates of different brain regions. FASEB Journal, 9(12), 1205-1210.