Melatonin protects against taurolithocholic-induced oxidative stress in rat liver

Lorena Fuentes-Broto, Francisco J. Miana-Mena, Eduardo Piedrafita, César Berzosa, Enrique Martínez-Ballarín, Francisco A. García-Gil, Russel J Reiter, Joaquín J. García

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Cholestasis, encountered in a variety of clinical disorders, is characterized by intracellular accumulation of toxic bile acids in the liver. Furthermore, oxidative stress plays an important role in the pathogenesis of bile acids. Taurolithocholic acid (TLC) was revealed in previous studies as the most pro-oxidative bile acid. Melatonin, a well-known antioxidant, is a safe and widely used therapeutic agent. Herein, we investigated the hepatoprotective role of melatonin on lipid and protein oxidation induced by TLC alone and in combination with FeCl3 and ascorbic acid in rat liver homogenates and hepatic membranes. The lipid peroxidation products, malondialdehyde and 4-hydroxyalkenals (MDA + 4-HDA), and carbonyl levels were quantified as indices of oxidative damage to hepatic lipids and proteins, respectively. In the current study, the rise in MDA + 4-HDA levels induced by TLC was inhibited by melatonin in a concentration-dependent manner in both liver homogenates and in hepatic membranes. Melatonin also had protective effects against structural damage to proteins induced by TLC in membranes. These results suggest that the indoleamine melatonin may potentially act as a protective agent in the therapy of those diseases that involve bile acid toxicity.

Original languageEnglish (US)
Pages (from-to)1219-1225
Number of pages7
JournalJournal of Cellular Biochemistry
Volume110
Issue number5
DOIs
StatePublished - Aug 1 2010

Fingerprint

Taurolithocholic Acid
Oxidative stress
Melatonin
Liver
Rats
Oxidative Stress
Bile Acids and Salts
Membranes
Lipids
Protective Agents
Proteins
Poisons
Malondialdehyde
Cholestasis
Ascorbic Acid
Toxicity
Antioxidants
Lipid Peroxidation
Oxidation
Therapeutics

Keywords

  • Bile acid
  • Cholestasis
  • Lipid peroxidation
  • Melatonin
  • Oxidative stress
  • Protein carbonyls
  • Taurolithocholic acid

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Fuentes-Broto, L., Miana-Mena, F. J., Piedrafita, E., Berzosa, C., Martínez-Ballarín, E., García-Gil, F. A., ... García, J. J. (2010). Melatonin protects against taurolithocholic-induced oxidative stress in rat liver. Journal of Cellular Biochemistry, 110(5), 1219-1225. https://doi.org/10.1002/jcb.22636

Melatonin protects against taurolithocholic-induced oxidative stress in rat liver. / Fuentes-Broto, Lorena; Miana-Mena, Francisco J.; Piedrafita, Eduardo; Berzosa, César; Martínez-Ballarín, Enrique; García-Gil, Francisco A.; Reiter, Russel J; García, Joaquín J.

In: Journal of Cellular Biochemistry, Vol. 110, No. 5, 01.08.2010, p. 1219-1225.

Research output: Contribution to journalArticle

Fuentes-Broto, L, Miana-Mena, FJ, Piedrafita, E, Berzosa, C, Martínez-Ballarín, E, García-Gil, FA, Reiter, RJ & García, JJ 2010, 'Melatonin protects against taurolithocholic-induced oxidative stress in rat liver', Journal of Cellular Biochemistry, vol. 110, no. 5, pp. 1219-1225. https://doi.org/10.1002/jcb.22636
Fuentes-Broto L, Miana-Mena FJ, Piedrafita E, Berzosa C, Martínez-Ballarín E, García-Gil FA et al. Melatonin protects against taurolithocholic-induced oxidative stress in rat liver. Journal of Cellular Biochemistry. 2010 Aug 1;110(5):1219-1225. https://doi.org/10.1002/jcb.22636
Fuentes-Broto, Lorena ; Miana-Mena, Francisco J. ; Piedrafita, Eduardo ; Berzosa, César ; Martínez-Ballarín, Enrique ; García-Gil, Francisco A. ; Reiter, Russel J ; García, Joaquín J. / Melatonin protects against taurolithocholic-induced oxidative stress in rat liver. In: Journal of Cellular Biochemistry. 2010 ; Vol. 110, No. 5. pp. 1219-1225.
@article{40d239fa340245f4b0cdd5176b100fe6,
title = "Melatonin protects against taurolithocholic-induced oxidative stress in rat liver",
abstract = "Cholestasis, encountered in a variety of clinical disorders, is characterized by intracellular accumulation of toxic bile acids in the liver. Furthermore, oxidative stress plays an important role in the pathogenesis of bile acids. Taurolithocholic acid (TLC) was revealed in previous studies as the most pro-oxidative bile acid. Melatonin, a well-known antioxidant, is a safe and widely used therapeutic agent. Herein, we investigated the hepatoprotective role of melatonin on lipid and protein oxidation induced by TLC alone and in combination with FeCl3 and ascorbic acid in rat liver homogenates and hepatic membranes. The lipid peroxidation products, malondialdehyde and 4-hydroxyalkenals (MDA + 4-HDA), and carbonyl levels were quantified as indices of oxidative damage to hepatic lipids and proteins, respectively. In the current study, the rise in MDA + 4-HDA levels induced by TLC was inhibited by melatonin in a concentration-dependent manner in both liver homogenates and in hepatic membranes. Melatonin also had protective effects against structural damage to proteins induced by TLC in membranes. These results suggest that the indoleamine melatonin may potentially act as a protective agent in the therapy of those diseases that involve bile acid toxicity.",
keywords = "Bile acid, Cholestasis, Lipid peroxidation, Melatonin, Oxidative stress, Protein carbonyls, Taurolithocholic acid",
author = "Lorena Fuentes-Broto and Miana-Mena, {Francisco J.} and Eduardo Piedrafita and C{\'e}sar Berzosa and Enrique Mart{\'i}nez-Ballar{\'i}n and Garc{\'i}a-Gil, {Francisco A.} and Reiter, {Russel J} and Garc{\'i}a, {Joaqu{\'i}n J.}",
year = "2010",
month = "8",
day = "1",
doi = "10.1002/jcb.22636",
language = "English (US)",
volume = "110",
pages = "1219--1225",
journal = "Journal of Cellular Biochemistry",
issn = "0730-2312",
publisher = "Wiley-Liss Inc.",
number = "5",

}

TY - JOUR

T1 - Melatonin protects against taurolithocholic-induced oxidative stress in rat liver

AU - Fuentes-Broto, Lorena

AU - Miana-Mena, Francisco J.

AU - Piedrafita, Eduardo

AU - Berzosa, César

AU - Martínez-Ballarín, Enrique

AU - García-Gil, Francisco A.

AU - Reiter, Russel J

AU - García, Joaquín J.

PY - 2010/8/1

Y1 - 2010/8/1

N2 - Cholestasis, encountered in a variety of clinical disorders, is characterized by intracellular accumulation of toxic bile acids in the liver. Furthermore, oxidative stress plays an important role in the pathogenesis of bile acids. Taurolithocholic acid (TLC) was revealed in previous studies as the most pro-oxidative bile acid. Melatonin, a well-known antioxidant, is a safe and widely used therapeutic agent. Herein, we investigated the hepatoprotective role of melatonin on lipid and protein oxidation induced by TLC alone and in combination with FeCl3 and ascorbic acid in rat liver homogenates and hepatic membranes. The lipid peroxidation products, malondialdehyde and 4-hydroxyalkenals (MDA + 4-HDA), and carbonyl levels were quantified as indices of oxidative damage to hepatic lipids and proteins, respectively. In the current study, the rise in MDA + 4-HDA levels induced by TLC was inhibited by melatonin in a concentration-dependent manner in both liver homogenates and in hepatic membranes. Melatonin also had protective effects against structural damage to proteins induced by TLC in membranes. These results suggest that the indoleamine melatonin may potentially act as a protective agent in the therapy of those diseases that involve bile acid toxicity.

AB - Cholestasis, encountered in a variety of clinical disorders, is characterized by intracellular accumulation of toxic bile acids in the liver. Furthermore, oxidative stress plays an important role in the pathogenesis of bile acids. Taurolithocholic acid (TLC) was revealed in previous studies as the most pro-oxidative bile acid. Melatonin, a well-known antioxidant, is a safe and widely used therapeutic agent. Herein, we investigated the hepatoprotective role of melatonin on lipid and protein oxidation induced by TLC alone and in combination with FeCl3 and ascorbic acid in rat liver homogenates and hepatic membranes. The lipid peroxidation products, malondialdehyde and 4-hydroxyalkenals (MDA + 4-HDA), and carbonyl levels were quantified as indices of oxidative damage to hepatic lipids and proteins, respectively. In the current study, the rise in MDA + 4-HDA levels induced by TLC was inhibited by melatonin in a concentration-dependent manner in both liver homogenates and in hepatic membranes. Melatonin also had protective effects against structural damage to proteins induced by TLC in membranes. These results suggest that the indoleamine melatonin may potentially act as a protective agent in the therapy of those diseases that involve bile acid toxicity.

KW - Bile acid

KW - Cholestasis

KW - Lipid peroxidation

KW - Melatonin

KW - Oxidative stress

KW - Protein carbonyls

KW - Taurolithocholic acid

UR - http://www.scopus.com/inward/record.url?scp=77954918018&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77954918018&partnerID=8YFLogxK

U2 - 10.1002/jcb.22636

DO - 10.1002/jcb.22636

M3 - Article

VL - 110

SP - 1219

EP - 1225

JO - Journal of Cellular Biochemistry

JF - Journal of Cellular Biochemistry

SN - 0730-2312

IS - 5

ER -