Melatonin protects against lipid peroxidation induced by δ- aminolevulinic acid in rat cerebellum, cortex and hippocampus

R. C.G. Carneiro, R. J. Reiter

Research output: Contribution to journalArticle

56 Scopus citations

Abstract

The in vitro and in vivo effect of melatonin on δ-aminolevulinic acid- induced lipid peroxidation in rat cerebellum, cortex and hipppocampus was determined. The concentration of malonaldehyde and 4-hydroxyalkenals was assayed as an index of induced membrane oxidative damage. The rise in malonaldehyde+4-hydroxyalkenals concentrations induced by δ-aminolevulinic acid in cerebellar homogenates was concentration-dependent (P<0.001) and also time-dependent in cerebellar, cortical and hippocampal homogenates (P<0.01). In vitro melatonin and vitamin E protected, in a concentration-dependent manner, against δ-aminolevulinic acid-induced lipid peroxidation in cortical, cerebellar and hippocampal homogenates. In in vivo experiments it was demonstrated that δ-aminolevulinic acid-induced lipid peroxidation (40 mg/kg) in cerebellum and hippocampus was reduced by acute melatonin (10 mg/kg) treatment (P<0.05). The results show that both in vitro and in vivo melatonin confers protection against δ-aminolevulinic acid-induced oxidative toxicity in brain regions. The findings suggest that melatonin may be useful in reducing neural damage in individuals suffering from acute intermittent porphyria.

Original languageEnglish (US)
Pages (from-to)293-299
Number of pages7
JournalNeuroscience
Volume82
Issue number1
DOIs
StatePublished - Sep 25 1997

Keywords

  • Brain
  • Free radicals
  • In vitro
  • In vivo
  • Melatonin
  • Porphyria

ASJC Scopus subject areas

  • Neuroscience(all)

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