Melatonin prevents changes in microsomal membrane fluidity during induced lipid peroxidation

Joaquín J. García; Russel J. Reiter; Juan M. Guerrero; Germaine Escames; Byung P. Yu; Chang S. Oh; Antonio Muñoz-Hoyos

doi:10.1016/S0014-5793(97)00447-X

Melatonin prevents changes in microsomal membrane fluidity during induced lipid peroxidation

Joaquín J. García, Russel J. Reiter, Juan M. Guerrero, Germaine Escames, Byung P. Yu, Chang S. Oh, Antonio Muñoz-Hoyos

Department of Cell Systems & Anatomy

Research output: Contribution to journal › Article › peer-review

315 Scopus citations

Abstract

We tested the effect of melatonin on membrane fluidity in microsomes of a rat liver model in which lipid peroxidation was induced by the addition of FeCl₃, ADP and NADPH. Membrane fluidity was monitored using fluorescence spectroscopy and lipid peroxidation,vas estimated by quantifying malonaldehyde (MDA)+4-hydroxyalkenals (4-HDA) concentrations following the induction of lipid peroxidation with and without pre-incubation with melatonin (1 μM-3 mM). Membrane rigidity increased during induced lipid peroxidation while melatonin reduced in a concentration-dependent manner both membrane rigidity and MDA+4-HDA generation. Melatonin's protective effect may relate to its known ability to scavenge free radicals and function as an antioxidant.

Original language	English (US)
Pages (from-to)	297-300
Number of pages	4
Journal	FEBS Letters
Volume	408
Issue number	3
DOIs	https://doi.org/10.1016/S0014-5793(97)00447-X
State	Published - May 26 1997

Keywords

Antioxidant
Free radical
Lipid peroxidation
Melatonin
Membrane fluidity
Microsome

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(97)00447-X

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title = "Melatonin prevents changes in microsomal membrane fluidity during induced lipid peroxidation",

abstract = "We tested the effect of melatonin on membrane fluidity in microsomes of a rat liver model in which lipid peroxidation was induced by the addition of FeCl3, ADP and NADPH. Membrane fluidity was monitored using fluorescence spectroscopy and lipid peroxidation,vas estimated by quantifying malonaldehyde (MDA)+4-hydroxyalkenals (4-HDA) concentrations following the induction of lipid peroxidation with and without pre-incubation with melatonin (1 μM-3 mM). Membrane rigidity increased during induced lipid peroxidation while melatonin reduced in a concentration-dependent manner both membrane rigidity and MDA+4-HDA generation. Melatonin's protective effect may relate to its known ability to scavenge free radicals and function as an antioxidant.",

keywords = "Antioxidant, Free radical, Lipid peroxidation, Melatonin, Membrane fluidity, Microsome",

author = "Garc{\'i}a, {Joaqu{\'i}n J.} and Reiter, {Russel J.} and Guerrero, {Juan M.} and Germaine Escames and Yu, {Byung P.} and Oh, {Chang S.} and Antonio Mu{\~n}oz-Hoyos",

note = "Funding Information: J.J.G. was supported by the University of Zaragoza (Spain). ",

year = "1997",

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doi = "10.1016/S0014-5793(97)00447-X",

language = "English (US)",

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T1 - Melatonin prevents changes in microsomal membrane fluidity during induced lipid peroxidation

AU - García, Joaquín J.

AU - Reiter, Russel J.

AU - Guerrero, Juan M.

AU - Escames, Germaine

AU - Yu, Byung P.

AU - Oh, Chang S.

AU - Muñoz-Hoyos, Antonio

N1 - Funding Information: J.J.G. was supported by the University of Zaragoza (Spain).

PY - 1997/5/26

Y1 - 1997/5/26

N2 - We tested the effect of melatonin on membrane fluidity in microsomes of a rat liver model in which lipid peroxidation was induced by the addition of FeCl3, ADP and NADPH. Membrane fluidity was monitored using fluorescence spectroscopy and lipid peroxidation,vas estimated by quantifying malonaldehyde (MDA)+4-hydroxyalkenals (4-HDA) concentrations following the induction of lipid peroxidation with and without pre-incubation with melatonin (1 μM-3 mM). Membrane rigidity increased during induced lipid peroxidation while melatonin reduced in a concentration-dependent manner both membrane rigidity and MDA+4-HDA generation. Melatonin's protective effect may relate to its known ability to scavenge free radicals and function as an antioxidant.

AB - We tested the effect of melatonin on membrane fluidity in microsomes of a rat liver model in which lipid peroxidation was induced by the addition of FeCl3, ADP and NADPH. Membrane fluidity was monitored using fluorescence spectroscopy and lipid peroxidation,vas estimated by quantifying malonaldehyde (MDA)+4-hydroxyalkenals (4-HDA) concentrations following the induction of lipid peroxidation with and without pre-incubation with melatonin (1 μM-3 mM). Membrane rigidity increased during induced lipid peroxidation while melatonin reduced in a concentration-dependent manner both membrane rigidity and MDA+4-HDA generation. Melatonin's protective effect may relate to its known ability to scavenge free radicals and function as an antioxidant.

KW - Antioxidant

KW - Free radical

KW - Lipid peroxidation

KW - Melatonin

KW - Membrane fluidity

KW - Microsome

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AN - SCOPUS:0031587041

SN - 0014-5793

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EP - 300

JO - FEBS Letters

JF - FEBS Letters

IS - 3

ER -

Melatonin prevents changes in microsomal membrane fluidity during induced lipid peroxidation

Abstract

Keywords

ASJC Scopus subject areas

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