Melatonin prevents δ-aminolevulinic acid-induced oxidative DNA damage in the presence of Fe2+

W. Qi, R. J. Reiter, D. X. Tan, L. C. Manchester, J. R. Calvo

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

δ-aminolevulinic acid (ALA), a heme precursor which accumulates during lead poisoning and acute intermittent porphyria, is reported to cause liver cancer. The carcinogenic mechanisms of ALA may relate to its ability to generate free radicals through metal-catalyzed oxidation which cause oxidative DNA damage. The aim of this study was to compare the efficacy of melatonin, trolox (vitamin E) and mannitol in altering DNA damage induced by ALA. Herein, we found, in the presence of Fe2+, that ALA-induced formation of 8-hydroxydeoxyguanosine in calf thymus DNA was dose and time-dependent. Melatonin, mannitol and trolox, all of which are free radical scavengers, inhibited the formation of 8-hydroxydeoxyguanosine in a concentration-dependent manner. The concentration of each (melatonin, mannitol and trolox) required to reduce DNA damage by 50%, i,e., the IC50, was 0.52, 0.84 and 0.90 mM, respectively.

Original languageEnglish (US)
Pages (from-to)87-92
Number of pages6
JournalMolecular and Cellular Biochemistry
Volume218
Issue number1-2
DOIs
StatePublished - Apr 24 2001

Keywords

  • 8-hydroxydeoxyguanosine
  • Mannitol
  • Melatonin
  • Vitamin E
  • δ-aminolevulinic acid

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

Fingerprint Dive into the research topics of 'Melatonin prevents δ-aminolevulinic acid-induced oxidative DNA damage in the presence of Fe<sup>2+</sup>'. Together they form a unique fingerprint.

  • Cite this