Melatonin mediates the stabilization of della proteins to repress the floral transition in Arabidopsis

Haitao Shi, Yunxie Wei, Qiannan Wang, Russel J Reiter, Chaozu He

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Precise floral transition from vegetative growth phase to reproductive growth phase is very important in plant life cycle. In flowering genetic pathways, DELLA proteins are master transcriptional regulators of gibberelic acid (GA) pathway, and FLOWERING LOCUS C (FLC) is a core repressor of vernalization pathway as well as downstream of DELLAs. As a crucial messenger in plants, the possible involvement of melatonin (N-acetyl-5-methoxytryptamine) in flowering and underlying molecular mechanism are unknown in Arabidopsis. In this study, we found that exogenous melatonin treatment delayed floral transition in Arabidopsis. Exogenous melatonin treatment conferred protein stabilizations of DELLAs [REPRESSOR of ga1-3 (RGA) and RGA-LIKE3 (RGL3)], without regulating the transcripts of DELLAs and endogenous GA level. Notably, exogenous melatonin delayed plant flowering and DELLA-activated transcripts of FLC were alleviated in della mutants, and those were exacerbated in DELLA overexpressing plants. Taken together, this study provides direct link between melatonin and floral transition, and indicates the novel involvement of DELLAs-activated FLC in melatonin-mediated flowering in Arabidopsis.

Original languageEnglish (US)
Pages (from-to)373-379
Number of pages7
JournalJournal of Pineal Research
Volume60
Issue number3
DOIs
StatePublished - Apr 1 2016

Fingerprint

Melatonin
Arabidopsis
Proteins
5-Methoxytryptamine
Acids
Growth
Life Cycle Stages

Keywords

  • Arabidopsis
  • DELLA
  • FLC
  • floral transition
  • gibberelic acid
  • Melatonin

ASJC Scopus subject areas

  • Endocrinology

Cite this

Melatonin mediates the stabilization of della proteins to repress the floral transition in Arabidopsis. / Shi, Haitao; Wei, Yunxie; Wang, Qiannan; Reiter, Russel J; He, Chaozu.

In: Journal of Pineal Research, Vol. 60, No. 3, 01.04.2016, p. 373-379.

Research output: Contribution to journalArticle

Shi, Haitao ; Wei, Yunxie ; Wang, Qiannan ; Reiter, Russel J ; He, Chaozu. / Melatonin mediates the stabilization of della proteins to repress the floral transition in Arabidopsis. In: Journal of Pineal Research. 2016 ; Vol. 60, No. 3. pp. 373-379.
@article{d714d4e65d2947a492a60c9640a809f3,
title = "Melatonin mediates the stabilization of della proteins to repress the floral transition in Arabidopsis",
abstract = "Precise floral transition from vegetative growth phase to reproductive growth phase is very important in plant life cycle. In flowering genetic pathways, DELLA proteins are master transcriptional regulators of gibberelic acid (GA) pathway, and FLOWERING LOCUS C (FLC) is a core repressor of vernalization pathway as well as downstream of DELLAs. As a crucial messenger in plants, the possible involvement of melatonin (N-acetyl-5-methoxytryptamine) in flowering and underlying molecular mechanism are unknown in Arabidopsis. In this study, we found that exogenous melatonin treatment delayed floral transition in Arabidopsis. Exogenous melatonin treatment conferred protein stabilizations of DELLAs [REPRESSOR of ga1-3 (RGA) and RGA-LIKE3 (RGL3)], without regulating the transcripts of DELLAs and endogenous GA level. Notably, exogenous melatonin delayed plant flowering and DELLA-activated transcripts of FLC were alleviated in della mutants, and those were exacerbated in DELLA overexpressing plants. Taken together, this study provides direct link between melatonin and floral transition, and indicates the novel involvement of DELLAs-activated FLC in melatonin-mediated flowering in Arabidopsis.",
keywords = "Arabidopsis, DELLA, FLC, floral transition, gibberelic acid, Melatonin",
author = "Haitao Shi and Yunxie Wei and Qiannan Wang and Reiter, {Russel J} and Chaozu He",
year = "2016",
month = "4",
day = "1",
doi = "10.1111/jpi.12320",
language = "English (US)",
volume = "60",
pages = "373--379",
journal = "Journal of Pineal Research",
issn = "0742-3098",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Melatonin mediates the stabilization of della proteins to repress the floral transition in Arabidopsis

AU - Shi, Haitao

AU - Wei, Yunxie

AU - Wang, Qiannan

AU - Reiter, Russel J

AU - He, Chaozu

PY - 2016/4/1

Y1 - 2016/4/1

N2 - Precise floral transition from vegetative growth phase to reproductive growth phase is very important in plant life cycle. In flowering genetic pathways, DELLA proteins are master transcriptional regulators of gibberelic acid (GA) pathway, and FLOWERING LOCUS C (FLC) is a core repressor of vernalization pathway as well as downstream of DELLAs. As a crucial messenger in plants, the possible involvement of melatonin (N-acetyl-5-methoxytryptamine) in flowering and underlying molecular mechanism are unknown in Arabidopsis. In this study, we found that exogenous melatonin treatment delayed floral transition in Arabidopsis. Exogenous melatonin treatment conferred protein stabilizations of DELLAs [REPRESSOR of ga1-3 (RGA) and RGA-LIKE3 (RGL3)], without regulating the transcripts of DELLAs and endogenous GA level. Notably, exogenous melatonin delayed plant flowering and DELLA-activated transcripts of FLC were alleviated in della mutants, and those were exacerbated in DELLA overexpressing plants. Taken together, this study provides direct link between melatonin and floral transition, and indicates the novel involvement of DELLAs-activated FLC in melatonin-mediated flowering in Arabidopsis.

AB - Precise floral transition from vegetative growth phase to reproductive growth phase is very important in plant life cycle. In flowering genetic pathways, DELLA proteins are master transcriptional regulators of gibberelic acid (GA) pathway, and FLOWERING LOCUS C (FLC) is a core repressor of vernalization pathway as well as downstream of DELLAs. As a crucial messenger in plants, the possible involvement of melatonin (N-acetyl-5-methoxytryptamine) in flowering and underlying molecular mechanism are unknown in Arabidopsis. In this study, we found that exogenous melatonin treatment delayed floral transition in Arabidopsis. Exogenous melatonin treatment conferred protein stabilizations of DELLAs [REPRESSOR of ga1-3 (RGA) and RGA-LIKE3 (RGL3)], without regulating the transcripts of DELLAs and endogenous GA level. Notably, exogenous melatonin delayed plant flowering and DELLA-activated transcripts of FLC were alleviated in della mutants, and those were exacerbated in DELLA overexpressing plants. Taken together, this study provides direct link between melatonin and floral transition, and indicates the novel involvement of DELLAs-activated FLC in melatonin-mediated flowering in Arabidopsis.

KW - Arabidopsis

KW - DELLA

KW - FLC

KW - floral transition

KW - gibberelic acid

KW - Melatonin

UR - http://www.scopus.com/inward/record.url?scp=84960172369&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84960172369&partnerID=8YFLogxK

U2 - 10.1111/jpi.12320

DO - 10.1111/jpi.12320

M3 - Article

VL - 60

SP - 373

EP - 379

JO - Journal of Pineal Research

JF - Journal of Pineal Research

SN - 0742-3098

IS - 3

ER -