@article{9753dccf5d9c4781a0b111c39a5f30b2,
title = "Melatonin inhibits seed germination by crosstalk with abscisic acid, gibberellin, and auxin in Arabidopsis",
abstract = "Seed germination, an important developmental stage in the life cycle of seed plants, is regulated by complex signals. Melatonin is a signaling molecule associated with seed germination under stressful conditions, although the underlying regulatory mechanisms are largely unknown. In this study, we showed that a low concentration (10 µM or 100 µM) of melatonin had no effect on seed germination, but when the concentration of melatonin increased to 500 µM or 1000 µM, seed germination was significantly inhibited in Arabidopsis. RNA sequencing analysis showed that melatonin regulated seed germination correlated to phytohormones abscisic acid (ABA), gibberellin (GA), and auxin. Further investigation revealed that ABA and melatonin synergistically inhibited seed germination, while GA and auxin antagonized the inhibitory effect of seed germination by melatonin. Disruption of the melatonin biosynthesis enzyme gene serotonin N-acetyltransferase (SNAT) or N-acetylserotonin methyltransferase (ASMT) promoted seed germination, while overexpression of ASMT inhibited seed germination. Taken together, our study sheds new light on the function and mechanism of melatonin in modulating seed germination in Arabidopsis.",
keywords = "N-acetylserotonin methyltransferase, Serotonin N-acetyltransferase, abscisic acid, auxin, gibberellin, melatonin, seed germination",
author = "Yan Lv and Jinjing Pan and Houping Wang and Reiter, {Russel J.} and Xia Li and Zongmin Mou and Jiemei Zhang and Zhengping Yao and Dake Zhao and Diqiu Yu",
note = "Funding Information: We thank Dr Jiafu Jiang (Nanjing Agricultural University), Dr Qi Chen (Kunming University of Science and Technology), and Dr Xingliang Hou (South China Botanical Garden, Chinese Academy of Sciences) for sharing research materials. This work was supported by the Natural Science Foundation of China (grants 31960082 to Dake Zhao; 31801023 to Houping Wang; 31671274 to Diqiu Yu; 32060136 to Jinjing Pan), the Yunnan Applied Basic Research Projects (grants 202001AW070009 to Houping Wang; 202001AU070125 to Jinjing Pan); Young Elite Scientists Sponsorship Program by CAST (2017QNRC001 to Houping Wang), the Youth Innovation Promotion Association CAS (to Houping Wang). We also thank Lawrence Ji from University of Pittsburgh and Dr Edward J. Kennelly from City University of New York for editing the English in this paper. Funding Information: We thank Dr Jiafu Jiang (Nanjing Agricultural University), Dr Qi Chen (Kunming University of Science and Technology), and Dr Xingliang Hou (South China Botanical Garden, Chinese Academy of Sciences) for sharing research materials. This work was supported by the Natural Science Foundation of China (grants 31960082 to Dake Zhao; 31801023 to Houping Wang; 31671274 to Diqiu Yu; 32060136 to Jinjing Pan), the Yunnan Applied Basic Research Projects (grants 202001AW070009 to Houping Wang; 202001AU070125 to Jinjing Pan); Young Elite Scientists Sponsorship Program by CAST (2017QNRC001 to Houping Wang), the Youth Innovation Promotion Association CAS (to Houping Wang). We also thank Lawrence Ji from University of Pittsburgh and Dr Edward J. Kennelly from City University of New York for editing the English in this paper. Publisher Copyright: {\textcopyright} 2021 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd",
year = "2021",
doi = "10.1111/jpi.12736",
language = "English (US)",
journal = "Journal of Pineal Research",
issn = "0742-3098",
publisher = "Wiley-Blackwell",
}