Melatonin induces browning of inguinal white adipose tissue in Zucker diabetic fatty rats

Aroa Jiménez-Aranda, Gumersindo Fernández-Vázquez, Daniel Campos, Mohamed Tassi, Lourdes Velasco-Perez, Dun Xian Tan, Russel J Reiter, Ahmad Agil

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

Melatonin limits obesity in rodents without affecting food intake and activity, suggesting a thermogenic effect. Identification of brown fat (beige/brite) in white adipose tissue (WAT) prompted us to investigate whether melatonin is a brown-fat inducer. We used Zücker diabetic fatty (ZDF) rats, a model of obesity-related type 2 diabetes and a strain in which melatonin reduces obesity and improves their metabolic profiles. At 5 wk of age, ZDF rats and lean littermates (ZL) were subdivided into two groups, each composed of four rats: control and those treated with oral melatonin in the drinking water (10 mg/kg/day) for 6 wk. Melatonin induced browning of inguinal WAT in both ZDF and ZL rats. Hematoxylin-eosin staining showed patches of brown-like adipocytes in inguinal WAT in ZDF rats and also increased the amounts in ZL animals. Inguinal skin temperature was similar in untreated lean and obese rats. Melatonin increased inguinal temperature by 1.36 ± 0.02°C in ZL and by 0.55 ± 0.04°C in ZDF rats and sensitized the thermogenic effect of acute cold exposure in both groups. Melatonin increased the amounts of thermogenic proteins, uncoupling protein 1 (UCP1) (by ~2-fold, P < 0.01) and PGC-1α (by 25%, P < 0.05) in extracts from beige inguinal areas in ZL rats. Melatonin also induced measurable amounts of UCP1 and stimulated by ~2-fold the levels of PGC-1α in ZDF animals. Locomotor activity and circulating irisin levels were not affected by melatonin. These results demonstrate that chronic oral melatonin drives WAT into a brown-fat-like function in ZDF rats. This may contribute to melatonin′s control of body weight and its metabolic benefits.

Original languageEnglish (US)
Pages (from-to)416-423
Number of pages8
JournalJournal of Pineal Research
Volume55
Issue number4
DOIs
StatePublished - Nov 2013

Fingerprint

White Adipose Tissue
Groin
Melatonin
Brown Adipose Tissue
Obesity
Brown Adipocytes
Metabolome
Skin Temperature
Hematoxylin
Locomotion
Eosine Yellowish-(YS)
Drinking Water
Type 2 Diabetes Mellitus
Rodentia
Eating
Body Weight
Staining and Labeling

Keywords

  • beige/brite adipose tissue
  • melatonin
  • mitochondria
  • nonshivering thermogenesis
  • obesity
  • white adipose tissue
  • ZDF rats

ASJC Scopus subject areas

  • Endocrinology

Cite this

Jiménez-Aranda, A., Fernández-Vázquez, G., Campos, D., Tassi, M., Velasco-Perez, L., Tan, D. X., ... Agil, A. (2013). Melatonin induces browning of inguinal white adipose tissue in Zucker diabetic fatty rats. Journal of Pineal Research, 55(4), 416-423. https://doi.org/10.1111/jpi.12089

Melatonin induces browning of inguinal white adipose tissue in Zucker diabetic fatty rats. / Jiménez-Aranda, Aroa; Fernández-Vázquez, Gumersindo; Campos, Daniel; Tassi, Mohamed; Velasco-Perez, Lourdes; Tan, Dun Xian; Reiter, Russel J; Agil, Ahmad.

In: Journal of Pineal Research, Vol. 55, No. 4, 11.2013, p. 416-423.

Research output: Contribution to journalArticle

Jiménez-Aranda, A, Fernández-Vázquez, G, Campos, D, Tassi, M, Velasco-Perez, L, Tan, DX, Reiter, RJ & Agil, A 2013, 'Melatonin induces browning of inguinal white adipose tissue in Zucker diabetic fatty rats', Journal of Pineal Research, vol. 55, no. 4, pp. 416-423. https://doi.org/10.1111/jpi.12089
Jiménez-Aranda A, Fernández-Vázquez G, Campos D, Tassi M, Velasco-Perez L, Tan DX et al. Melatonin induces browning of inguinal white adipose tissue in Zucker diabetic fatty rats. Journal of Pineal Research. 2013 Nov;55(4):416-423. https://doi.org/10.1111/jpi.12089
Jiménez-Aranda, Aroa ; Fernández-Vázquez, Gumersindo ; Campos, Daniel ; Tassi, Mohamed ; Velasco-Perez, Lourdes ; Tan, Dun Xian ; Reiter, Russel J ; Agil, Ahmad. / Melatonin induces browning of inguinal white adipose tissue in Zucker diabetic fatty rats. In: Journal of Pineal Research. 2013 ; Vol. 55, No. 4. pp. 416-423.
@article{fa91cc6339d343cbbdbb8840823f6dcc,
title = "Melatonin induces browning of inguinal white adipose tissue in Zucker diabetic fatty rats",
abstract = "Melatonin limits obesity in rodents without affecting food intake and activity, suggesting a thermogenic effect. Identification of brown fat (beige/brite) in white adipose tissue (WAT) prompted us to investigate whether melatonin is a brown-fat inducer. We used Z{\"u}cker diabetic fatty (ZDF) rats, a model of obesity-related type 2 diabetes and a strain in which melatonin reduces obesity and improves their metabolic profiles. At 5 wk of age, ZDF rats and lean littermates (ZL) were subdivided into two groups, each composed of four rats: control and those treated with oral melatonin in the drinking water (10 mg/kg/day) for 6 wk. Melatonin induced browning of inguinal WAT in both ZDF and ZL rats. Hematoxylin-eosin staining showed patches of brown-like adipocytes in inguinal WAT in ZDF rats and also increased the amounts in ZL animals. Inguinal skin temperature was similar in untreated lean and obese rats. Melatonin increased inguinal temperature by 1.36 ± 0.02°C in ZL and by 0.55 ± 0.04°C in ZDF rats and sensitized the thermogenic effect of acute cold exposure in both groups. Melatonin increased the amounts of thermogenic proteins, uncoupling protein 1 (UCP1) (by ~2-fold, P < 0.01) and PGC-1α (by 25{\%}, P < 0.05) in extracts from beige inguinal areas in ZL rats. Melatonin also induced measurable amounts of UCP1 and stimulated by ~2-fold the levels of PGC-1α in ZDF animals. Locomotor activity and circulating irisin levels were not affected by melatonin. These results demonstrate that chronic oral melatonin drives WAT into a brown-fat-like function in ZDF rats. This may contribute to melatonin′s control of body weight and its metabolic benefits.",
keywords = "beige/brite adipose tissue, melatonin, mitochondria, nonshivering thermogenesis, obesity, white adipose tissue, ZDF rats",
author = "Aroa Jim{\'e}nez-Aranda and Gumersindo Fern{\'a}ndez-V{\'a}zquez and Daniel Campos and Mohamed Tassi and Lourdes Velasco-Perez and Tan, {Dun Xian} and Reiter, {Russel J} and Ahmad Agil",
year = "2013",
month = "11",
doi = "10.1111/jpi.12089",
language = "English (US)",
volume = "55",
pages = "416--423",
journal = "Journal of Pineal Research",
issn = "0742-3098",
publisher = "Wiley-Blackwell",
number = "4",

}

TY - JOUR

T1 - Melatonin induces browning of inguinal white adipose tissue in Zucker diabetic fatty rats

AU - Jiménez-Aranda, Aroa

AU - Fernández-Vázquez, Gumersindo

AU - Campos, Daniel

AU - Tassi, Mohamed

AU - Velasco-Perez, Lourdes

AU - Tan, Dun Xian

AU - Reiter, Russel J

AU - Agil, Ahmad

PY - 2013/11

Y1 - 2013/11

N2 - Melatonin limits obesity in rodents without affecting food intake and activity, suggesting a thermogenic effect. Identification of brown fat (beige/brite) in white adipose tissue (WAT) prompted us to investigate whether melatonin is a brown-fat inducer. We used Zücker diabetic fatty (ZDF) rats, a model of obesity-related type 2 diabetes and a strain in which melatonin reduces obesity and improves their metabolic profiles. At 5 wk of age, ZDF rats and lean littermates (ZL) were subdivided into two groups, each composed of four rats: control and those treated with oral melatonin in the drinking water (10 mg/kg/day) for 6 wk. Melatonin induced browning of inguinal WAT in both ZDF and ZL rats. Hematoxylin-eosin staining showed patches of brown-like adipocytes in inguinal WAT in ZDF rats and also increased the amounts in ZL animals. Inguinal skin temperature was similar in untreated lean and obese rats. Melatonin increased inguinal temperature by 1.36 ± 0.02°C in ZL and by 0.55 ± 0.04°C in ZDF rats and sensitized the thermogenic effect of acute cold exposure in both groups. Melatonin increased the amounts of thermogenic proteins, uncoupling protein 1 (UCP1) (by ~2-fold, P < 0.01) and PGC-1α (by 25%, P < 0.05) in extracts from beige inguinal areas in ZL rats. Melatonin also induced measurable amounts of UCP1 and stimulated by ~2-fold the levels of PGC-1α in ZDF animals. Locomotor activity and circulating irisin levels were not affected by melatonin. These results demonstrate that chronic oral melatonin drives WAT into a brown-fat-like function in ZDF rats. This may contribute to melatonin′s control of body weight and its metabolic benefits.

AB - Melatonin limits obesity in rodents without affecting food intake and activity, suggesting a thermogenic effect. Identification of brown fat (beige/brite) in white adipose tissue (WAT) prompted us to investigate whether melatonin is a brown-fat inducer. We used Zücker diabetic fatty (ZDF) rats, a model of obesity-related type 2 diabetes and a strain in which melatonin reduces obesity and improves their metabolic profiles. At 5 wk of age, ZDF rats and lean littermates (ZL) were subdivided into two groups, each composed of four rats: control and those treated with oral melatonin in the drinking water (10 mg/kg/day) for 6 wk. Melatonin induced browning of inguinal WAT in both ZDF and ZL rats. Hematoxylin-eosin staining showed patches of brown-like adipocytes in inguinal WAT in ZDF rats and also increased the amounts in ZL animals. Inguinal skin temperature was similar in untreated lean and obese rats. Melatonin increased inguinal temperature by 1.36 ± 0.02°C in ZL and by 0.55 ± 0.04°C in ZDF rats and sensitized the thermogenic effect of acute cold exposure in both groups. Melatonin increased the amounts of thermogenic proteins, uncoupling protein 1 (UCP1) (by ~2-fold, P < 0.01) and PGC-1α (by 25%, P < 0.05) in extracts from beige inguinal areas in ZL rats. Melatonin also induced measurable amounts of UCP1 and stimulated by ~2-fold the levels of PGC-1α in ZDF animals. Locomotor activity and circulating irisin levels were not affected by melatonin. These results demonstrate that chronic oral melatonin drives WAT into a brown-fat-like function in ZDF rats. This may contribute to melatonin′s control of body weight and its metabolic benefits.

KW - beige/brite adipose tissue

KW - melatonin

KW - mitochondria

KW - nonshivering thermogenesis

KW - obesity

KW - white adipose tissue

KW - ZDF rats

UR - http://www.scopus.com/inward/record.url?scp=84885619152&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84885619152&partnerID=8YFLogxK

U2 - 10.1111/jpi.12089

DO - 10.1111/jpi.12089

M3 - Article

VL - 55

SP - 416

EP - 423

JO - Journal of Pineal Research

JF - Journal of Pineal Research

SN - 0742-3098

IS - 4

ER -