Melatonin improves deferoxamine antioxidant activity in protecting against lipid peroxidation caused by hydrogen peroxide in rat brain homogenates

S. Ortega-Gutiérrez, J. J. García, E. Martínez-Ballarín, R. J. Reiter, S. Millán-Plano, M. Robinson, D. Acua-Castroviejo

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Deferoxamine (DF) is an antioxidant molecule because of its ability to chelate iron. This study compared the ability of DF alone or in combination with melatonin, 5-methoxytryptophol or pinoline in preventing lipid peroxidation due to hydrogen peroxide (H2O2) in rat brain homogenates. Malondialdehyde (MDA) and 4-hydroxyalkenals (4-HDA) in the homogenates were measured as indices of lipid peroxidation. Incubation of homogenates with DF reduced, in a dose-dependent manner, MDA + 4-HDA formation due to H2O2. When melatonin, 5-methoxytryptophol or pinoline were added to the incubation medium, the efficacy of DF in preventing lipid peroxidation was enhanced. These cooperative effects between DF, melatonin, and related pineal products may be important in protecting tissues from the oxidative stress due to iron overload.

Original languageEnglish (US)
Pages (from-to)55-59
Number of pages5
JournalNeuroscience Letters
Volume323
Issue number1
DOIs
StatePublished - Apr 19 2002

Keywords

  • Brain homogenates
  • Deferoxamine
  • Hydrogen peroxide
  • Lipid peroxidation
  • Melatonin

ASJC Scopus subject areas

  • Neuroscience(all)

Fingerprint Dive into the research topics of 'Melatonin improves deferoxamine antioxidant activity in protecting against lipid peroxidation caused by hydrogen peroxide in rat brain homogenates'. Together they form a unique fingerprint.

Cite this