Melatonin enhances the occurrence of autophagy induced by oxidative stress in Arabidopsis seedlings

Ping Wang, Xun Sun, Na Wang, Dun Xian Tan, Fengwang Ma

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The beneficial effect that melatonin has against mitochondrial dysfunctioning seems to be linked to mitophagy. Roles for melatonin have been demonstrated in promoting health and preventing disease, as well as activating the process of autophagy in general. However, no reports have been made about how the application of melatonin regulates that process when plants are exposed to oxidative stress. We investigated the influence of different concentrations of melatonin (0.0, 0.5, 5.0, 10.0, or 50.0 μm) on autophagy under methyl viologen (MV)-induced oxidative stress. Arabidopsis seedlings that were pretreated with 5 or 10 μm melatonin underwent relatively strong induction of autophagy, as evidenced by the number of monodansylcadaverine (MDC)-stained autophagosomes in root samples. Pretreatment with 10 μm melatonin also alleviated MV-induced photo-oxidation damage and significantly reduced the accumulation of oxidized proteins. Those responses might have been due to the strong upregulation of genes that involved in AtATG8-PE conjugation pathway, which enhanced the capacity for autophagy. Histochemical staining revealed that both O2-· and H<inf>2</inf>O<inf>2</inf> were highly accumulated upon MV exposure, although the response did not differ significantly between control and melatonin-pretreated seedlings. By contrast, exogenous melatonin upregulated the expression of two genes for H<inf>2</inf>O<inf>2</inf>-scavenging enzymes, that is, AtAPX1 and AtCATs. The activation of autophagy by melatonin without an alteration in ROS production may be part of a survival mechanism that is enhanced by melatonin after cellular damage. Therefore, it represents a second level of defense to remove damaged proteins when antioxidant activities are compromised.

Original languageEnglish (US)
Pages (from-to)479-489
Number of pages11
JournalJournal of Pineal Research
Volume58
Issue number4
DOIs
StatePublished - May 1 2015

Fingerprint

Autophagy
Melatonin
Seedlings
Arabidopsis
Oxidative Stress
Paraquat
Mitochondrial Degradation
Proteins
Up-Regulation
Antioxidants
Staining and Labeling
Gene Expression

Keywords

  • Arabidopsis
  • AtATG8s
  • autophagy
  • melatonin
  • oxidative stress
  • oxidized protein

ASJC Scopus subject areas

  • Endocrinology

Cite this

Melatonin enhances the occurrence of autophagy induced by oxidative stress in Arabidopsis seedlings. / Wang, Ping; Sun, Xun; Wang, Na; Tan, Dun Xian; Ma, Fengwang.

In: Journal of Pineal Research, Vol. 58, No. 4, 01.05.2015, p. 479-489.

Research output: Contribution to journalArticle

Wang, Ping ; Sun, Xun ; Wang, Na ; Tan, Dun Xian ; Ma, Fengwang. / Melatonin enhances the occurrence of autophagy induced by oxidative stress in Arabidopsis seedlings. In: Journal of Pineal Research. 2015 ; Vol. 58, No. 4. pp. 479-489.
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